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Blockade of CHRNB2 signaling with a therapeutic monoclonal antibody attenuates the aggressiveness of gastric cancer cells

Blockade of CHRNB2 signaling with a therapeutic monoclonal antibody attenuates the aggressiveness of gastric cancer cells

Posted on August 28, 2021September 4, 2021 by Cody

Here, we evaluated the therapeutic potential of antibodies (Abs) focusing on cholinergic receptor nicotinic beta 2 subunit (CHRNB2) in gastric cancer. To examine the results of these Abs on malignant phenotypes in vitro and in mouse xenograft fashions, we generated gene knockouts by way of genome enhancing, carried out RNA interference-mediated knockdown of gene expression, and ectopically expressed CHRNB2 in gastric cancer cells. The results of anti-CHRNB2 Abs on the proliferation of cancer cells had been evaluated each in vitro and in vivo. We decided the results of Chrnb2 deficiency on mice and the medical significance of CHRNB2 expression in gastric cancer medical specimens.

Knockdown of CHRNB2 attenuated gastric cancer cell proliferation, whereas compelled overexpression of CHRNB2 elevated cell proliferation. Knockout of CHRNB2 considerably influenced cell survival and capabilities related with metastasis. The results of polyclonal Abs focusing on the C- and N-termini of CHRNB2 guided the improvement of anti-CHRNB2 monoclonal Abs that inhibited the development of gastric cancer cells in vitro and in vivo. Pathway evaluation revealed that CHRNB2 interfered with signaling by way of the PI3K-AKT and JAK-STAT pathways. Chrnb2-deficient mice exhibited regular replica, organ capabilities, and motor capabilities. CHRNB2 regulates a number of oncological phenotypes related with metastasis, and blockade of CHRNB2 expression utilizing particular Abs exhibits promise for controlling metastasis in gastric cancer.

About 25% of sufferers with IgA nephropathy (IgAN) progress to stage 5 persistent kidney illness (CKD) after years of evolution. Various instruments have been developed in recent times designed to foretell which of the sufferers will had poorer outcomes. The worth of circulating galactosyl-deficient IgA1 (Gd-IgA1) has been associated to a worse evolution of IgAN in a number of research. There are additionally some publications that relate greater APRIL values with a worse evolution. Recently, a new technique has been developed that enables measuring the worth of circulating Gd-IgA1 in a less complicated manner than these beforehand obtainable. The goal of this research is to investigate the affect of circulating Gd-IgA1, measured by this technique, on the development of IgAN.

Monoclonal Antibody Targeting the CD154 Cleavage Site Inhibits CD40-Dependent and -Independent Cleavage of CD154 from the Cell Surface

In addition to the membrane-bound molecule, soluble CD154 (sCD154) can also be detected at excessive ranges in the medium of activated T cells and platelets and in the serum of sufferers affected by completely different inflammatory ailments. This sCD154 is the consequence of cleavage of the full-length molecule between the glutamic acid residue at place 112 (E112) and methionine at place 113 (M113) and will be derived from the intracellular milieu and from cleavage of cell floor molecules. We have just lately reported that substitution of each E112 and M113 by alanine inhibits intracellular and CD40-induced membrane cleavage of CD154 and procures to CD154 an elevated organic operate as in contrast with cleavable CD154.

Blockade of CHRNB2 signaling with a therapeutic monoclonal antibody attenuates the aggressiveness of gastric cancer cells

Thus, on this research, and in the intention of growing instruments inhibiting cleavage of CD154 from the cell floor, we generated a panel of anti-human CD154 mAbs. One of the derived mAbs that didn’t alter the binding of sCD154 to CD40, named on this research Clone eight mAb, completely misplaced its binding exercise in opposition to cells expressing CD154 mutated at its E112 and M113 residues. Treatment with Clone eight mAb was proven to fully abolish CD40-dependent and -independent cleavage of CD154 from the cell floor. Our research is highlighting the improvement and characterization of an modern therapeutic device succesful of inhibiting the launch/cleavage of CD154 from cells and thus sustaining its availability on the cell floor and the excessive in all probability of growing its efficiency as an activator of CD40-induced responses.

In this quasi-experimental pre-/postimplementation research, we estimated the effectiveness of MAb remedy inside 7 days of symptom onset in high-risk ambulatory adults with COVID-19. The main consequence was a composite of emergency division visits or hospitalizations inside 14 days of optimistic check. Secondary outcomes included adversarial occasions and 14-day mortality. The common remedy impact in the handled for MAb remedy was estimated utilizing inverse chance of remedy weighting and the affect of MAb implementation utilizing propensity-weighted interrupted time sequence evaluation.

Electrostatic Spray Drying for Monoclonal Antibody Formulation

This research explored the feasibility of electrostatic spray drying for producing a monoclonal antibody (mAb) powder formulation at decrease drying temperatures than typical spray drying and its impact on protein stability. A mAb formulation was dried by both typical spray drying or electrostatic spray drying with cost (ESD). The protein powders had been then characterised utilizing solid-state Fourier remodel infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), dimension exclusion chromatography (SEC), and solid-state hydrogen/deuterium alternate with mass spectrometry (ssHDX-MS).

Particle characterizations reminiscent of BET floor space, particle dimension distribution, and particle morphology had been additionally carried out. Conventional spray drying of the mAb formulation at the inlet temperature of 70°C didn’t generate dry powders as a consequence of poor drying effectivity; electrostatic spray drying at the similar temperature at 5kV enabled the formation of powder formulation with passable moisture contents. Deconvoluted peak areas of deuterated samples from the ssHDX-MS research confirmed a good correlation with the loss of the monomeric peak space measured by dimension exclusion chromatography in the 90-day accelerated stability research carried out at 40°C.

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID047AF Fitzgerald 500 ug
EUR 462
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated

S0013 Affbiotech 200ul
EUR 304

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated

S0018 Affbiotech 200ul
EUR 304

Goat anti Mouse IgG1 (Alexa Fluor 488)

43R-1649 Fitzgerald 500 ug
EUR 570
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)

43R-ID028AF Fitzgerald 500 ug
EUR 430
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-ID056AF Fitzgerald 500 ug
EUR 343
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594)

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-IR016AF Fitzgerald 1 mg
EUR 281
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)

50337 Alpha Diagnostics 0.5 ml
EUR 225

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated

Ab8032-001 GenDepot 0.5mg
EUR 435

AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]

16892 AAT Bioquest 1 mg
EUR 176

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]

23158 AAT Bioquest 300 Tests
EUR 306

Anti-RPSA Alexa Fluor® 488

A4-829-C100 ExBio 0.1 mg
EUR 310

Anti-CD40 antibody (Alexa-fluor 488)

STJ170000 St John's Laboratory 100 µg
EUR 393
Description: CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40L (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40, predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin

Anti-LAMP3 antibody (Alexa-fluor 488)

STJ170004 St John's Laboratory 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 546)

STJ170005 St John's Laboratory 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 647)

STJ170006 St John's Laboratory 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 488)

STJ170009 St John's Laboratory 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 546)

STJ170010 St John's Laboratory 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 647)

STJ170011 St John's Laboratory 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 488)

STJ170014 St John's Laboratory 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 546)

STJ170015 St John's Laboratory 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 647)

STJ170016 St John's Laboratory 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 488)

STJ170020 St John's Laboratory 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 546)

STJ170021 St John's Laboratory 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 647)

STJ170022 St John's Laboratory 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Endoglin/CD105 Alexa Fluor

FC15024 Neuromics 100 Tests
EUR 448

Anti-Hu CD16 Alexa Fluor® 488

A4-646-T100 ExBio 100 tests
EUR 269

EGFR antibody (Alexa Fluor 488)

61R-E109BAF Fitzgerald 125 ug
EUR 706
Description: Mouse monoclonal EGFR antibody (Alexa Fluor 488)

SAM FCM (Alexa Fluor 647)

abx098902-100tests Abbexa 100 tests
EUR 1233

SAM FCM (Alexa Fluor 488)

abx098904-60tests Abbexa 60 tests
EUR 1358

DyLight®594 Conjugated Goat Anti-mouse IgG (H+L)

BA1141-0.5 BosterBio 0.5ml
EUR 202

DyLight®594 Conjugated Goat Anti-mouse IgG (H+L)

BA1141-1 BosterBio 1ml
EUR 334

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)

50336 Alpha Diagnostics 0.5 ml
EUR 225

DyLight®594 Conjugated Goat Anti-rabbit IgG (H+L)

BA1142-0.5 BosterBio 0.5ml
EUR 202

DyLight®594 Conjugated Goat Anti-rabbit IgG (H+L)

BA1142-1 BosterBio 1ml
EUR 334

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)

43R-ID060AF Fitzgerald 300 ug
EUR 425
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647)

Anti-Hu CD72 Alexa Fluor® 488

A4-310-T100 ExBio 100 tests
EUR 269

Anti-Bov CD9 Alexa Fluor® 488

A4-354-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD30 Alexa Fluor® 700

A7-455-T100 ExBio 100 tests
EUR 269

Anti-Hu CD94 Alexa Fluor® 700

A7-727-T100 ExBio 100 tests
EUR 269

Anti-Hu CD56 Alexa Fluor® 700

A7-789-T100 ExBio 100 tests
EUR 269

iFluor™ 594 goat anti-mouse IgG (H+L)

16468 AAT Bioquest 200 ug
EUR 115

iFluor™ 594 goat anti-mouse IgG (H+L)

16741 AAT Bioquest 1 mg
EUR 176

Goat Anti-Mouse IgG (H+L) Antibody (DyLight 594)

20-abx007449 Abbexa
  • EUR 272.00
  • EUR 453.00
  • 100 ul
  • 500 ul

Alpha Fluor™ 594 C5 Maleimide

1891 AAT Bioquest 1 mg
EUR 219

Streptavidin-Alexa594 (Alexas fluor 594) conjugate

SV-A594-100 Alpha Diagnostics 100 tests
EUR 225

Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]

1795 AAT Bioquest 10 mg
EUR 393

Goat anti-Mouse IgG SecondaryAntibody (peroxidase conjugated)

E0L3032-1 EnoGene 500ul
EUR 128

Polyclonal Goat anti-GST α-form

GST-ANTI-1 Detroit R&D 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 Detroit R&D 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 Detroit R&D 50 uL
EUR 280

iFluor™ 594 goat anti-rabbit IgG (H+L)

16628 AAT Bioquest 200 ug
EUR 115

iFluor™ 594 goat anti-rabbit IgG (H+L)

16806 AAT Bioquest 1 mg
EUR 176

Rabbit Anti-Goat IgG (H+L) Antibody (DyLight 594)

20-abx007407 Abbexa
  • EUR 272.00
  • EUR 453.00
  • 100 ul
  • 500 ul

Goat Anti-Rat IgG (H+L) Antibody (DyLight 594)

20-abx007419 Abbexa
  • EUR 272.00
  • EUR 453.00
  • 100 ul
  • 500 ul

Goat Anti-Rabbit IgG (H+L) Antibody (DyLight 594)

20-abx007433 Abbexa
  • EUR 272.00
  • EUR 453.00
  • 100 ul
  • 500 ul

Goat anti Mouse IgG

20-B9015G000-S0 Fitzgerald 10 ml
EUR 133
Description: Goat anti Mouse IgG antibody

Goat anti Mouse IgG

20-S8301G000-V0 Fitzgerald 10 ml
EUR 133
Description: Goat anti Mouse IgG antibody

Goat anti Mouse IgG

41-GM25 Fitzgerald 20 mg
EUR 184
Description: Goat anti Mouse IgG secondary antibody

Goat anti Mouse IgG

41R-1058 Fitzgerald 2 mg
EUR 149
Description: Goat anti Mouse IgG secondary antibody

Goat anti Mouse IgG

40-GM25S Fitzgerald 100 ml
EUR 133
Description: Goat anti Mouse IgG secondary antibody

Goat anti Mouse IgG

40-S8301G000-S4 Fitzgerald 10 ml
EUR 133
Description: Goat anti Mouse IgG secondary antibody

Goat Anti Mouse IgG

E61I00501 EnoGene 100ug
EUR 343

Goat Anti-mouse IgG

STJ15100178 St John's Laboratory 250 µg
EUR 283
Description: Biotinylated goat anti-mouse IgG enables detection of mouse IgG in enzyme immunoassays such as indirect ELISA.

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488

A4-686-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488

A4-712-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488

A4-730-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488

A4-737-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647

A6-686-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647

A6-712-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647

A6-730-C100 ExBio 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647

A6-737-C100 ExBio 0.1 mg
EUR 269

iFluor™ 594 goat anti-mouse IgG (H+L) *Cross Adsorbed*

16548 AAT Bioquest 200 ug
EUR 132

iFluor™ 594 goat anti-mouse IgG (H+L) *Cross Adsorbed*

16780 AAT Bioquest 1 mg
EUR 219

Goat anti Mouse IgG (H + L) (Agarose Conjugated)

43R-IG044AS Fitzgerald 20 mg
EUR 488
Description: Goat anti Mouse IgG (H + L) secondary antibody (Agarose Conjugated)

Goat Anti-Mouse IgG(H+L) FITC –conjugated

S0007 Affbiotech 200ul
EUR 376

Goat Anti-Mouse IgG(H+L) CY3–conjugated

S0012 Affbiotech 200ul
EUR 304

Goat Anti-Mouse IgG(H+L) TRITC–conjugated

S0016 Affbiotech 200ul
EUR 304

HRP-conjugated Goat Anti-Mouse IgG Heavy Chain

AS064 Abclonal 100 ul
EUR 152

Goat anti-Mouse IgG(H+L), Biotin Conjugated

E16SPB101-100 EnoGene 100Tests
EUR 343

Goat anti-Mouse IgG(H+L), Biotin Conjugated

E16SPB101-1000 EnoGene 1000Tests
EUR 245

Goat anti-Mouse IgG(H+L), Biotin Conjugated

E16SPB101-500 EnoGene 500Tests
EUR 167

Goat anti-Mouse IgG(H+L), FITC Conjugated

E16SPF101-050 EnoGene 50Tests
EUR 239

Goat anti-Mouse IgG(H+L), FITC Conjugated

E16SPF101-100 EnoGene 100Tests
EUR 343

Goat anti-Mouse IgG(H+L), FITC Conjugated

E16SPF101-100U EnoGene 100μg
EUR 239

Goat anti-Mouse IgG(H+L), FITC Conjugated

E16SPF101-500U EnoGene 500μg
EUR 343

Goat anti-Mouse IgG(H+L) ,HRPO Conjugated

E16SPH101-100 EnoGene 100μl
EUR 239

Goat anti-Mouse IgG(H+L) ,HRPO Conjugated

E16SPH101-1000 EnoGene 1000μl
EUR 434

Goat anti-Mouse IgG(H+L) ,HRPO Conjugated

E16SPH101-500 EnoGene 500μl
EUR 343

Goat anti-Mouse IgG(H+L), PE Conjugated

E16SPP101-020 EnoGene 20Tests
EUR 239

Goat anti-Mouse IgG(H+L), PE Conjugated

E16SPP101-050 EnoGene 50Tests
EUR 239

Goat anti-Mouse IgG(H+L), PE Conjugated

E16SPP101-100U EnoGene 100ug
EUR 239

Goat anti-Mouse IgG(H+L), PE Conjugated

E16SPP101-500U EnoGene 500ug
EUR 343

Goat Anti-Mouse IgG Secondary Antibody AF790 Conjugated

L30310 SAB 0.5 ml
EUR 126

Goat Anti-Mouse IgG Secondary Antibody Cy3 Conjugated

L30311 SAB 1.0 ml
EUR 112

Goat Anti-Mouse IgG Secondary Antibody FITC Conjugated

L30313 SAB 1.0 ml
EUR 97

Goat Anti-Mouse IgG Secondary Antibody APC Conjugated

L30316 SAB 0.5 ml
EUR 191

Goat Anti-Mouse IgG Secondary Antibody PE Conjugated

L30317 SAB 1.0 ml
EUR 191

Goat Anti-Mouse IgG Secondary Antibody PerCP Conjugated

L30318 SAB 0.5 ml
EUR 191

Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated

L3032 SAB 1.0 ml
EUR 112

Goat Anti-Mouse IgG Secondary Antibody AP Conjugated

L3033 SAB 1.0 ml
EUR 119

Goat Anti-Mouse IgG Secondary Antibody Biotin Conjugated

L3034 SAB 1.0 ml
EUR 104

Goat Anti-Mouse IgG Secondary Antibody AMCA Conjugated

L3035 SAB 1.0 ml
EUR 97

Goat Anti-Mouse IgG Secondary Antibody AF488 Conjugated

L3036 SAB 1.0 ml
EUR 112

Goat Anti-Mouse IgG Secondary Antibody AF594 Conjugated

L3037 SAB 1.0 ml
EUR 112

Goat Anti-Mouse IgG Secondary Antibody AF647 Conjugated

L3038 SAB 1.0 ml
EUR 112

Goat Anti-Mouse IgG Secondary Antibody AF680 Conjugated

L3039 SAB 0.5 ml
EUR 126

AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]

16890 AAT Bioquest 1 mg
EUR 176

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]

16891 AAT Bioquest 1 mg
EUR 176

AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin]

23150 AAT Bioquest 300 Tests
EUR 306

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]

23153 AAT Bioquest 300 Tests
EUR 306

Goat Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-g-48T DL Develop 48T
EUR 464
Description: A competitive inhibition quantitative ELISA assay kit for detection of Goat Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Goat Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-g-96T DL Develop 96T
EUR 601
Description: A competitive inhibition quantitative ELISA assay kit for detection of Goat Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Goat Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-g-48Tests Reddot Biotech 48 Tests
EUR 482

Goat Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-g-96Tests Reddot Biotech 96 Tests
EUR 667

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488)

M09406 BosterBio 100ug
EUR 565
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Goat anti Mouse IgG + IgM

41R-1063 Fitzgerald 1 mg
EUR 137
Description: Goat anti Mouse IgG + IgM secondary antibody

Goat anti Mouse IgG + IgM

41R-1064 Fitzgerald 1 mg
EUR 194
Description: Goat anti Mouse IgG + IgM secondary antibody

Goat anti Mouse IgG (HRP)

43C-CB1569 Fitzgerald 1 mg
EUR 198
Description: Goat anti Mouse IgG secondary antibody  (HRP)

Goat anti Mouse IgG (FITC)

43R-1359 Fitzgerald 2 mg
EUR 186
Description: Goat anti Mouse IgG secondary antibody  (FITC)

Goat anti Mouse IgG (rhodamine)

43R-1360 Fitzgerald 1 mg
EUR 138
Description: Goat anti Mouse IgG secondary antibody  (rhodamine)

Goat Anti-Mouse IgG -HRP

LF-SA8001 Abfrontier 0.5 ml
EUR 131
Description: Goat polyclonal to mouse IgG

Goat Anti-Mouse IgG -HRP

LF-SA8001A Abfrontier 1.0 ml
EUR 192
Description: Goat polyclonal to mouse IgG

Goat Anti-Mouse IgG-TRITC

LF-SA8001TR Abfrontier 1.0 mg
EUR 287
Description: Goat polyclonal to mouse IgG

Goat Anti-Mouse IgG/HRP

E3GAM001 EnoGene 200ul
EUR 408

Goat Anti Mouse IgG -Biotin

E61I00502 EnoGene 1mg
EUR 499

Goat Anti Mouse IgG-HRP

E61I00503 EnoGene 1mg
EUR 499

Goat Anti Mouse IgG-FITC

E61I00504 EnoGene 1mg
EUR 611

Goat Anti-mouse IgG-ALP

STJ15100031 St John's Laboratory 1 ml
EUR 350
Description: This ALP-conjugate enables detection of mouse IgG in enzyme immunoassays such as indirect ELISA and Western blot.

488 Goat-anti-Mouse IgG

GT26000 Neuromics 1 mg
EUR 375

546 Goat-anti-Mouse IgG

GT26003 Neuromics 1 mg
EUR 375

642 Goat-anti-Mouse IgG

GT26005 Neuromics 1 mg
EUR 375

Goat Anti-Mouse IgG (Fc)

GAMG80Fc-0100 Equitech 100ml
EUR 404.3

Goat Anti-Mouse IgG (Fc)

GAMG80Fc-0500 Equitech 500ml
EUR 707.2

Goat Anti-Mouse IgG (Fc) IgG, unlabeled

40151-UL Alpha Diagnostics 1 mg
EUR 213

Goat anti Human IgG (Fc) (Agarose Conjugated)

43R-IG016AS Fitzgerald 2 ml
EUR 381
Description: Goat anti Human IgG secondary antibody (Fc) (Agarose Conjugated)

Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated)

E0L3012-1 EnoGene 500ul
EUR 128

Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated)

E0L3012-2 EnoGene 1000ul
EUR 180

HRP Conjugated anti-Goat IgG SABC Kit

SA1023 BosterBio 1 kit
EUR 152

FITC Conjugated anti-Goat IgG SABC Kit

SA1066 BosterBio 1 kit
EUR 334

Cy3 Conjugated anti-Goat IgG SABC Kit

SA1076 BosterBio 1 kit
EUR 355

HRP Conjugated anti-Goat IgG SABC Kit

SA2003 BosterBio 1 kit
EUR 334

HRP-conjugated AffiniPure Goat Anti-Mouse IgG Light Chain

AS062 Abclonal 100 ul
EUR 152

Mouse anti Goat IgG heavy chain-HRP Conjugated Antibody

C48068 SAB 100ul
EUR 397

Mouse anti goat IgG lighty chain-HRP Conjugated Antibody

C48078 SAB 100ul
EUR 397

Goat Anti-Mouse IgG Secondary Antibody Rhodamine (TRITC) Conjugated

L30314 SAB 1.0 ml
EUR 97

Goat Anti-Mouse IgG Secondary Antibody Rhodamine (RRX) Conjugated

L30315 SAB 1.0 ml
EUR 97

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488)

M02316 BosterBio 100ug
EUR 580
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human.

iFluor™ 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*

16698 AAT Bioquest 200 ug
EUR 132

iFluor™ 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*

16833 AAT Bioquest 1 mg
EUR 219

Goat Anti-Mouse IgG (Fc) IgG-Biotin Conjugate

40151-BT Alpha Diagnostics 1 ml
EUR 225

Goat Anti-Mouse IgG (Fc) IgG-FITC Conjugate

40151-FITC Alpha Diagnostics 1 ml
EUR 225

Goat Anti-Mouse IgG (Fc) IgG-HRP Conjugate

40151-HRP Alpha Diagnostics 1 ml
EUR 225

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN ImmunoStep 50 µg
EUR 349

Ibrutinib drug-Human IgG Conjugate

IBT17-IgG Alpha Diagnostics 100 ug
EUR 529

Donkey Anti-Mouse IgG (H+L) Antibody (DyLight 594)

20-abx007461 Abbexa
  • EUR 314.00
  • EUR 592.00
  • 100 ul
  • 500 ul

Goat anti Mouse IgG (H + L)

41C-CB1501 Fitzgerald 2 mg
EUR 216
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (Fab'2)

41C-CJ0207 Fitzgerald 1 mg
EUR 298
Description: Goat anti Mouse IgG secondary antibody (Fab'2)

Goat anti Mouse IgG (Fab'2)

41C-CJ0213 Fitzgerald 1 mg
EUR 294
Description: Goat anti Mouse IgG secondary antibody (Fab'2)

Goat anti Mouse IgG (H + L)

41R-1051 Fitzgerald 2 mg
EUR 149
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (H + L)

41R-1052 Fitzgerald 2 mg
EUR 137
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (H + L)

41R-1053 Fitzgerald 2 mg
EUR 149
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (H + L)

41R-1054 Fitzgerald 2 mg
EUR 194
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (H + L)

41R-1055 Fitzgerald 2 mg
EUR 309
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (H + L)

41R-1056 Fitzgerald 1 mg
EUR 153
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (H + L)

41R-1057 Fitzgerald 1 mg
EUR 186
Description: Goat anti Mouse IgG (H + L) secondary antibody

Low-temperature (70°C inlet temperature) drying with an electrostatic cost (5kV) led to higher protein bodily stability as in contrast with the samples spray-dried at the excessive temperature (130°C inlet temperature) with out cost. This research exhibits that electrostatic spray drying can produce strong monoclonal antibody formulation at decrease inlet temperature than conventional spray drying with higher bodily stability.

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Categories

  • 4 Host
  • 96T
  • apoptosis
  • array
  • Assay
  • Bacteria
  • Blocking
  • Combination of pembrolizumab and 125I attenuates the aggressiveness of non-small cell lung cancer.
  • Comparison of Efficacy of Topical Curcumin Gel with Triamcinolone-hyaluronidase Gel Individually and in Combination in the Treatment of Oral Submucous Fibrosis.
  • Comprehensive analysis of the long non-coding RNA-associated competitive endogenous RNA network reveals novel prognostic biomarkers in Wilms' tumor.
  • GMO
  • Goat
  • Increased expression of plakoglobin is associated with upregulated MAPK and PI3K/AKT signalling pathways in early resectable pancreatic ductal adenocarcinoma.
  • LINC00460 accelerates progression of ovarian cancer by activating transcriptional factor ZNF703.
  • Next-generation laser-based photodynamic endoscopic diagnosis using 5-aminolevulinic acid for early gastric adenocarcinoma and gastric adenoma.
  • Pig
  • Pigeon
  • Plant
  • plasmid
  • Plate
  • Prone en-bloc sacrectomy with proctectomy: A surgical approach to the inaccessible and hostile pelvis
  • Sleeve Gastrectomy: Surgical Technique, Outcomes, and Complications

Recent Posts

  • Pancreas-type ribonucleasen zijn
  • Rapid and comprehensive monoclonal antibody Characterization using microfluidic CE-MS
  • Orally administered anti-eotaxin-1 monoclonal antibody is biologically active in the gut and alleviates immune-mediated hepatitis: A novel anti-inflammatory personalized therapeutic approach
  • Preferential Light-Chain Labeling of Native Monoclonal Antibodies Improves the Properties of Fluorophore Conjugates
  • Application of tunable resistive pulse sensing for the quantification of submicron particles in pharmaceutical monoclonal antibody preparations

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Recent Posts

  • Pancreas-type ribonucleasen zijn
  • Rapid and comprehensive monoclonal antibody Characterization using microfluidic CE-MS
  • Orally administered anti-eotaxin-1 monoclonal antibody is biologically active in the gut and alleviates immune-mediated hepatitis: A novel anti-inflammatory personalized therapeutic approach
  • Preferential Light-Chain Labeling of Native Monoclonal Antibodies Improves the Properties of Fluorophore Conjugates
  • Application of tunable resistive pulse sensing for the quantification of submicron particles in pharmaceutical monoclonal antibody preparations
Breast Cancer Antibodies

Categories

  • 4 Host (6)
  • 96T (6)
  • apoptosis (6)
  • array (6)
  • Assay (5)
  • Bacteria (7)
  • Blocking (6)
  • Combination of pembrolizumab and 125I attenuates the aggressiveness of non-small cell lung cancer. (1)
  • Comparison of Efficacy of Topical Curcumin Gel with Triamcinolone-hyaluronidase Gel Individually and in Combination in the Treatment of Oral Submucous Fibrosis. (1)
  • Comprehensive analysis of the long non-coding RNA-associated competitive endogenous RNA network reveals novel prognostic biomarkers in Wilms' tumor. (1)
  • GMO (6)
  • Goat (6)
  • Increased expression of plakoglobin is associated with upregulated MAPK and PI3K/AKT signalling pathways in early resectable pancreatic ductal adenocarcinoma. (1)
  • LINC00460 accelerates progression of ovarian cancer by activating transcriptional factor ZNF703. (1)
  • Next-generation laser-based photodynamic endoscopic diagnosis using 5-aminolevulinic acid for early gastric adenocarcinoma and gastric adenoma. (1)
  • Pig (6)
  • Pigeon (6)
  • Plant (6)
  • plasmid (6)
  • Plate (5)
  • Prone en-bloc sacrectomy with proctectomy: A surgical approach to the inaccessible and hostile pelvis (1)
  • Sleeve Gastrectomy: Surgical Technique, Outcomes, and Complications (25)

Categories

  • 4 Host
  • 96T
  • apoptosis
  • array
  • Assay
  • Bacteria
  • Blocking
  • Combination of pembrolizumab and 125I attenuates the aggressiveness of non-small cell lung cancer.
  • Comparison of Efficacy of Topical Curcumin Gel with Triamcinolone-hyaluronidase Gel Individually and in Combination in the Treatment of Oral Submucous Fibrosis.
  • Comprehensive analysis of the long non-coding RNA-associated competitive endogenous RNA network reveals novel prognostic biomarkers in Wilms' tumor.
  • GMO
  • Goat
  • Increased expression of plakoglobin is associated with upregulated MAPK and PI3K/AKT signalling pathways in early resectable pancreatic ductal adenocarcinoma.
  • LINC00460 accelerates progression of ovarian cancer by activating transcriptional factor ZNF703.
  • Next-generation laser-based photodynamic endoscopic diagnosis using 5-aminolevulinic acid for early gastric adenocarcinoma and gastric adenoma.
  • Pig
  • Pigeon
  • Plant
  • plasmid
  • Plate
  • Prone en-bloc sacrectomy with proctectomy: A surgical approach to the inaccessible and hostile pelvis
  • Sleeve Gastrectomy: Surgical Technique, Outcomes, and Complications
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