Site particular labeling strategies have important potential to boost the properties of antibody conjugates. While studied extensively in the context of antibody-drug conjugates (ADCs), few research have examined the influence of homogenous labeling on the properties of antibody-fluorophore conjugates (AFCs). We report the utility of pentafluorophenyl (PFP) esters, which had beforehand been proven to be fairly selective for Ok188 of the kappa gentle chain of human IGG antibodies, towards producing AFCs. We present that easy substitute of N-hydroxy succinimide (NHS) with PFP dramatically will increase the light-chain specificity of near-infrared (NIR) AFCs. Comparing the properties of AFCs labeled utilizing NHS and PFP-activated esters reveals that the latter displays lowered aggregation and improved brightness, each in vitro and in vivo.
Overall, the use of PFP esters gives a remarkably easy strategy to offer selectively labeled antibodies with improved properties. Fully automated characterization of monoclonal antibody (mAb) cost variants utilizing four-dimensional liquid chromatography-mass spectrometry (4D-LC-MS) is reported and illustrated. Charge variants resolved by cation-exchange chromatography (CEX) utilizing a salt- or pH-gradient are collected in loops put in on a a number of heart-cutting valve and consequently subjected to on-line desalting, denaturation, discount and trypsin digestion previous to LC-MS primarily based peptide mapping.
This innovation which considerably reduces turnaround time, pattern manipulation, loss and artefacts and will increase data gathering, is described in nice technical element, and utilized to characterize the cost heterogeneity related to three therapeutic mAbs. Sequence coverages > 95% are obtained for main and minor cost variants (> 1.0%). Post-translational modifications (PTMs) and modification websites are readily revealed in a repeatable method together with unstable succinimide intermediates which aren’t maintained when performing classical in-solution in a single day digestion of offline collected CEX peaks.
A chondroitin sulfate proteoglycan 4‑particular monoclonal antibody inhibits melanoma cell invasion in a spheroid mannequin
The overexpression of chondroitin sulfate proteoglycan 4 (CSPG4) is related to a number of tumor sorts, together with malignant melanoma, squamous cell carcinoma, triple‑unfavorable breast carcinoma, oligodendrocytomas or gliomas. Due to its restricted distribution in regular tissues, CSPG4 has been thought-about a possible goal for a number of antitumor approaches, together with monoclonal antibody (mAb) therapies. The intention of the current examine was to characterize the influence of the CSPG4‑particular mAb clone 9.2.27 by itself or together with the generally used BRAF‑selective inhibitor, PLX4032, on completely different capabilities of melanoma cells to evaluate the potential synergistic results.
The BRAF V600‑mutant human melanoma cell strains, M14 (CSPG4‑unfavorable) and WM164 (CSPG4‑optimistic), have been uncovered to the CSPG4‑particular 9.2.27 mAb and/or PLX4032. Cell viability and colony formation capability have been evaluated. A 3D‑cell tradition spheroid mannequin was used to evaluate the invasive properties of the handled cells. In addition, stream cytometric evaluation of apoptosis and cell cycle analyses have been carried out. Incubation of the WM164 cell line with CSPG4‑particular 9.2.27 mAb decreased viability, colony formation skill and the invasive capability of CSPG4‑optimistic tumor cells, which was not the case for the CSPG4‑unfavorable M14 cell line. Combined therapy of the WM164 cells with 9.2.27 mAb plus PLX4032 didn’t exert any important further impact compared to therapy with PLX4032 alone in the clonogenic and invasion assays.
M14 cell cycle distribution was not influenced by the CSPG4‑particular 9.2.27 mAb. By distinction, the publicity of WM164 cells to the mAb resulted in an arrest of the cells in the S section. Moreover, mixed therapy of the WM164 cells led to a considerably elevated accumulation of cells in the subG1 section, mixed with a lower of cells in the G2/M section. On the entire, findings of the current examine point out that the CSPG4‑particular 9.2.27 mAb exerts an anti‑invasive impact on CSPG4‑optimistic melanoma spheroids, which isn’t enhanced by BRAF inhibition. These findings present the foundation for additional investigations on the results of anti‑CSPG4‑primarily based therapies of CSPG4‑optimistic tumors.
Pathogenic Role of Circulating Citrullinated Antigens and Anti-Cyclic Monoclonal Citrullinated Peptide Antibodies in Rheumatoid Arthritis
We examined whether or not it’s potential to immediately detect citrullinated antigens in the serum of rheumatoid arthritis (RA) sufferers utilizing a monoclonal antibody (mAb) designed to be particular for citrullinated peptides. In order to verify the potential of the mAb as a direct arthritis-inducing substance by experimental mannequin of RA, a monoclonal antibody (mAb) 12G1 was generated utilizing by immunization of mice with a difficult cyclic citrullinated peptide. Immunohistochemical evaluation of RA-affected synovial tissue confirmed that our mAb 12G1 may certainly detect citrullinated proteins in goal tissues.
Subsequently, serum ranges of citrullinated sort II collagen and filaggrin have been measured in wholesome volunteers, sufferers with RA, ankylosing spondylitis (AS), and systemic lupus erythematosus (SLE) utilizing a 12G1-based sandwich ELISA. This confirmed that citrullinated filaggrin confirmed 78.9% sensitivity and 85.9% specificity for RA analysis with a cutoff optical density (OD) worth of 1.013, comparable with the outcomes from a second-generation anti-citrullinated protein antibody (ACPA) check. Circulating citrullinated collagen and filaggrin have been detected even in sera of RA sufferers who have been unfavorable for each rheumatoid issue (RF) and ACPA.
 (Alexa Fluor 594)) Donkey anti Goat IgG (H + L) (Alexa Fluor 594) |
|
43R-ID005AF |
Fitzgerald |
500 ug |
EUR 338 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594) |
, Alexa Fluor® 594 Conjugated) Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated |
|
Ab8011-001 |
GenDepot |
1mg |
EUR 334 |
 Alexa Fluor 647–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated |
|
S0013 |
Affbiotech |
200ul |
EUR 304 |
 Alexa Fluor 488–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated |
|
S0018 |
Affbiotech |
200ul |
EUR 304 |
 (Fab 2) (Alexa Fluor 594)) Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594) |
|
43R-ID012AF |
Fitzgerald |
300 ug |
EUR 410 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594) |
 (Alexa Fluor 594)) Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
|
43R-ID022AF |
Fitzgerald |
500 ug |
EUR 364 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
|
43R-ID047AF |
Fitzgerald |
500 ug |
EUR 462 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
 Alexa Fluor 647–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated |
|
S0014 |
Affbiotech |
200ul |
EUR 304 |
 Alexa Fluor 488–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated |
|
S0017 |
Affbiotech |
200ul |
EUR 304 |
 (Alexa Fluor 594)) Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594) |
|
43R-IR016AF |
Fitzgerald |
1 mg |
EUR 281 |
|
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594) |
-Alexa 594 Fluor conjugate (adsorbed with human IgG)) Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG) |
|
50337 |
Alpha Diagnostics |
0.5 ml |
EUR 225 |
 (Alexa Fluor 647)) Donkey anti Goat IgG (H + L) (Alexa Fluor 647) |
|
43R-ID028AF |
Fitzgerald |
500 ug |
EUR 430 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647) |
, Alexa Fluor® 488 Conjugated) Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated |
|
Ab8032-001 |
GenDepot |
0.5mg |
EUR 435 |
) Goat anti Mouse IgG1 (Alexa Fluor 488) |
|
43R-1649 |
Fitzgerald |
500 ug |
EUR 570 |
|
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488) |
 (Alexa Fluor 594)) Donkey anti Chicken IgY (H + L) (Alexa Fluor 594) |
|
43R-ID056AF |
Fitzgerald |
500 ug |
EUR 343 |
|
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594) |
 AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate] |
|
16892 |
AAT Bioquest |
1 mg |
EUR 176 |
|
|
 AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin] |
|
23158 |
AAT Bioquest |
300 Tests |
EUR 306 |
|
|
 Anti-RPSA Alexa Fluor® 488 |
|
A4-829-C100 |
ExBio |
0.1 mg |
EUR 310 |
) Anti-CD40 antibody (Alexa-fluor 488) |
|
STJ170000 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40L (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40, predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin |
) Anti-LAMP3 antibody (Alexa-fluor 488) |
|
STJ170004 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
) Anti-LAMP3 antibody (Alexa-fluor 546) |
|
STJ170005 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
) Anti-LAMP3 antibody (Alexa-fluor 647) |
|
STJ170006 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
) Anti-IL3RA antibody (Alexa-fluor 488) |
|
STJ170009 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
) Anti-IL3RA antibody (Alexa-fluor 546) |
|
STJ170010 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
) Anti-IL3RA antibody (Alexa-fluor 647) |
|
STJ170011 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
) Anti-CD207 antibody (Alexa-fluor 488) |
|
STJ170014 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
) Anti-CD207 antibody (Alexa-fluor 546) |
|
STJ170015 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
) Anti-CD207 antibody (Alexa-fluor 647) |
|
STJ170016 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
) Anti-IL7R antibody (Alexa-fluor 488) |
|
STJ170020 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54) |
) Anti-IL7R antibody (Alexa-fluor 546) |
|
STJ170021 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54) |
) Anti-IL7R antibody (Alexa-fluor 647) |
|
STJ170022 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54) |
 Endoglin/CD105 Alexa Fluor |
|
FC15024 |
Neuromics |
100 Tests |
EUR 448 |
-Alexa 488 Fluor conjugate (adsorbed with human IgG)) Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG) |
|
50336 |
Alpha Diagnostics |
0.5 ml |
EUR 225 |
) DyLight®594 Conjugated Goat Anti-rabbit IgG (H+L) |
|
BA1142-0.5 |
BosterBio |
0.5ml |
EUR 202 |
DyLight®594 Conjugated Goat Anti-rabbit IgG (H+L) |
|
BA1142-1 |
BosterBio |
1ml |
EUR 334 |
 Anti-Hu CD16 Alexa Fluor® 488 |
|
A4-646-T100 |
ExBio |
100 tests |
EUR 269 |
) EGFR antibody (Alexa Fluor 488) |
|
61R-E109BAF |
Fitzgerald |
125 ug |
EUR 706 |
|
Description: Mouse monoclonal EGFR antibody (Alexa Fluor 488) |
) SAM FCM (Alexa Fluor 647) |
|
abx098902-100tests |
Abbexa |
100 tests |
EUR 1233 |
|
|
) SAM FCM (Alexa Fluor 488) |
|
abx098904-60tests |
Abbexa |
60 tests |
EUR 1358 |
|
|
) iFluor™ 594 goat anti-rabbit IgG (H+L) |
|
16628 |
AAT Bioquest |
200 ug |
EUR 115 |
|
|
iFluor™ 594 goat anti-rabbit IgG (H+L) |
|
16806 |
AAT Bioquest |
1 mg |
EUR 176 |
|
|
 Antibody (DyLight 594)) Rabbit Anti-Goat IgG (H+L) Antibody (DyLight 594) |
|
20-abx007407 |
Abbexa |
|
|
|
|
 Antibody (DyLight 594)) Goat Anti-Rabbit IgG (H+L) Antibody (DyLight 594) |
|
20-abx007433 |
Abbexa |
|
|
|
|
) DyLight®594 Conjugated Goat Anti-mouse IgG (H+L) |
|
BA1141-0.5 |
BosterBio |
0.5ml |
EUR 202 |
DyLight®594 Conjugated Goat Anti-mouse IgG (H+L) |
|
BA1141-1 |
BosterBio |
1ml |
EUR 334 |
 Rabbit Polyclonal antibody Anti-CRBN |
|
Anti-CRBN |
ImmunoStep |
50 µg |
EUR 349 |
 (Alexa Fluor 647)) Donkey anti Chicken IgY (H + L) (Alexa Fluor 647) |
|
43R-ID060AF |
Fitzgerald |
300 ug |
EUR 425 |
|
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647) |
 Anti-Hu CD72 Alexa Fluor® 488 |
|
A4-310-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Bov CD9 Alexa Fluor® 488 |
|
A4-354-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Anti-Hu CD30 Alexa Fluor® 700 |
|
A7-455-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD94 Alexa Fluor® 700 |
|
A7-727-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD56 Alexa Fluor® 700 |
|
A7-789-T100 |
ExBio |
100 tests |
EUR 269 |
) Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated) |
|
E0L3012-1 |
EnoGene |
500ul |
EUR 128 |
Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated) |
|
E0L3012-2 |
EnoGene |
1000ul |
EUR 180 |
 Rabbit anti Goat IgG |
|
20-S5801R000-VO |
Fitzgerald |
10 ml |
EUR 133 |
|
Description: Rabbit anti Goat IgG antibody |
Rabbit anti Goat IgG |
|
40C-CB0536 |
Fitzgerald |
1.5 mg |
EUR 269 |
|
Description: Rabbit anti Goat IgG secondary antibody |
 Goat anti Rabbit IgG |
|
40C-CB1157 |
Fitzgerald |
5 mg |
EUR 311 |
|
Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
|
41-GR30 |
Fitzgerald |
10 mg |
EUR 277 |
|
Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
|
41-GR31 |
Fitzgerald |
5 mg |
EUR 162 |
|
Description: Goat anti Rabbit IgG secondary antibody |
Rabbit anti Goat IgG |
|
41C-CB0529 |
Fitzgerald |
2 mg |
EUR 244 |
|
Description: Rabbit anti Goat IgG secondary antibody |
Goat anti Rabbit IgG |
|
41C-CB1143 |
Fitzgerald |
2 mg |
EUR 278 |
|
Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
|
41C-CB1150 |
Fitzgerald |
10 mg |
EUR 388 |
|
Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
|
41R-1079 |
Fitzgerald |
2 mg |
EUR 149 |
|
Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
|
40-GR30 |
Fitzgerald |
100 ml |
EUR 241 |
|
Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
|
40-GR30S |
Fitzgerald |
100 ml |
EUR 133 |
|
Description: Goat anti Rabbit IgG secondary antibody |
Rabbit anti Goat IgG |
|
E61I01002 |
EnoGene |
1mg |
EUR 343 |
 goat Anti Rabbit IgG |
|
E61I01301 |
EnoGene |
1mg |
EUR 274 |
 *Cross Adsorbed*) iFluor™ 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed* |
|
16698 |
AAT Bioquest |
200 ug |
EUR 132 |
|
|
iFluor™ 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed* |
|
16833 |
AAT Bioquest |
1 mg |
EUR 219 |
|
|
 Alpha Fluor™ 594 C5 Maleimide |
|
1891 |
AAT Bioquest |
1 mg |
EUR 219 |
|
|
 conjugate) Streptavidin-Alexa594 (Alexas fluor 594) conjugate |
|
SV-A594-100 |
Alpha Diagnostics |
100 tests |
EUR 225 |
 (Agarose Conjugated)) Goat anti Rabbit IgG (H + L) (Agarose Conjugated) |
|
43R-IG045AS |
Fitzgerald |
20 mg |
EUR 475 |
|
Description: Goat anti Rabbit IgG (H + L) secondary antibody (Agarose Conjugated) |
 (Agarose Conjugated)) Rabbit anti Goat IgG (H + L) (Agarose Conjugated) |
|
43R-IR004AS |
Fitzgerald |
20 mg |
EUR 541 |
|
Description: Rabbit anti Goat IgG (H + L) secondary antibody (Agarose Conjugated) |
 FITC–conjugated) Goat Anti-Rabbit IgG(H+L) FITC–conjugated |
|
S0008 |
Affbiotech |
200ul |
EUR 376 |
 CY3–conjugated) Goat Anti-Rabbit IgG(H+L) CY3–conjugated |
|
S0011 |
Affbiotech |
200ul |
EUR 304 |
 TRITC–conjugated) Goat Anti-Rabbit IgG(H+L) TRITC–conjugated |
|
S0015 |
Affbiotech |
200ul |
EUR 304 |
 HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain |
|
AS063 |
Abclonal |
100 ul |
EUR 152 |
, Biotin Conjugated) Goat anti- Rabbit IgG(H+L), Biotin Conjugated |
|
E16SPB102-100 |
EnoGene |
100Tests |
EUR 343 |
Goat anti- Rabbit IgG(H+L), Biotin Conjugated |
|
E16SPB102-1000 |
EnoGene |
1000Tests |
EUR 245 |
Goat anti- Rabbit IgG(H+L), Biotin Conjugated |
|
E16SPB102-500 |
EnoGene |
500Tests |
EUR 167 |
， FITC Conjugated) Goat anti-Rabbit IgG(H+L)， FITC Conjugated |
|
E16SPF102-050 |
EnoGene |
50Tests |
EUR 239 |
Goat anti-Rabbit IgG(H+L)， FITC Conjugated |
|
E16SPF102-100 |
EnoGene |
100Tests |
EUR 343 |
Goat anti-Rabbit IgG(H+L)， FITC Conjugated |
|
E16SPF102-100U |
EnoGene |
100μg |
EUR 239 |
Goat anti-Rabbit IgG(H+L)， FITC Conjugated |
|
E16SPF102-500U |
EnoGene |
500μg |
EUR 343 |
, HRPO Conjugated) Goat anti-Rabbit IgG(H+L), HRPO Conjugated |
|
E16SPH102-100 |
EnoGene |
100μl |
EUR 239 |
Goat anti-Rabbit IgG(H+L), HRPO Conjugated |
|
E16SPH102-1000 |
EnoGene |
1000μl |
EUR 434 |
Goat anti-Rabbit IgG(H+L), HRPO Conjugated |
|
E16SPH102-500 |
EnoGene |
500μl |
EUR 343 |
， PE Conjugated) Goat anti-Rabbit IgG(H+L)， PE Conjugated |
|
E16SPP102-050 |
EnoGene |
50Tests |
EUR 239 |
Goat anti-Rabbit IgG(H+L)， PE Conjugated |
|
E16SPP102-100 |
EnoGene |
100Tests |
EUR 343 |
Goat anti-Rabbit IgG(H+L)， PE Conjugated |
|
E16SPP102-100U |
EnoGene |
100ug |
EUR 239 |
Goat anti-Rabbit IgG(H+L)， PE Conjugated |
|
E16SPP102-500U |
EnoGene |
500ug |
EUR 343 |
 Goat Anti-Rabbit IgG Secondary Antibody Cy3 Conjugated |
|
L30111 |
SAB |
1.0 ml |
EUR 112 |
 Goat Anti-Rabbit IgG Secondary Antibody FITC Conjugated |
|
L30113 |
SAB |
1.0 ml |
EUR 97 |
 Goat Anti-Rabbit IgG Secondary Antibody APC Conjugated |
|
L30116 |
SAB |
0.5 ml |
EUR 191 |
 Goat Anti-Rabbit IgG Secondary Antibody PE Conjugated |
|
L30117 |
SAB |
1.0 ml |
EUR 191 |
 Goat Anti-Rabbit IgG Secondary Antibody PerCP Conjugated |
|
L30118 |
SAB |
0.5 ml |
EUR 191 |
 Goat Anti-Rabbit IgG Secondary Antibody HRP Conjugated |
|
L3012 |
SAB |
1.0 ml |
EUR 112 |
 Goat Anti-Rabbit IgG Secondary Antibody Biotin Conjugated |
|
L3014 |
SAB |
1.0 ml |
EUR 104 |
 Goat Anti-Rabbit IgG Secondary Antibody AF488 Conjugated |
|
L3016 |
SAB |
1.0 ml |
EUR 112 |
 Goat Anti-Rabbit IgG Secondary Antibody AF594 Conjugated |
|
L3017 |
SAB |
1.0 ml |
EUR 112 |
 Goat Anti-Rabbit IgG Secondary Antibody AF647 Conjugated |
|
L3018 |
SAB |
1.0 ml |
EUR 112 |
 Rabbit anti-Goat IgG Secondary Antibody HRP conjugated |
|
L3042 |
SAB |
1.0 ml |
EUR 112 |
 Goat Anti-Rabbit IgG Secondary Antibody AF680 Conjugated |
|
L3049 |
SAB |
0.5 ml |
EUR 126 |
 Goat Anti-Rabbit IgG Secondary Antibody AF790 Conjugated |
|
L30510 |
SAB |
0.5 ml |
EUR 126 |
 Goat Anti-Rabbit IgG Secondary Antibody AMCA Conjugated |
|
L3065 |
SAB |
1.0 ml |
EUR 97 |
 Goat Anti-Rabbit IgG Secondary Antibody AP Conjugated |
|
L3073 |
SAB |
1.0 ml |
EUR 119 |
 Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid] |
|
1795 |
AAT Bioquest |
10 mg |
EUR 393 |
|
|
 Goat anti Rabbit IgG Fc |
|
70R-IG011 |
Fitzgerald |
1.5 mg |
EUR 378 |
|
Description: Goat anti Rabbit IgG Fc secondary antibody |
Goat anti Rabbit IgG Fc |
|
70R-IG017 |
Fitzgerald |
2 mg |
EUR 381 |
|
Description: Goat anti Rabbit IgG Fc secondary antibody |
Goat anti Rabbit IgG Fc |
|
41C-CB1136 |
Fitzgerald |
2 mg |
EUR 249 |
|
Description: Goat anti Rabbit IgG secondary antibody |
 Rabbit anti Goat IgG Fc |
|
41R-1013 |
Fitzgerald |
1 mg |
EUR 188 |
|
Description: Rabbit anti Goat IgG Fc fragment secondary antibody |
Rabbit anti Goat IgG Fc |
|
41R-1014 |
Fitzgerald |
1 mg |
EUR 207 |
|
Description: Rabbit anti Goat IgG Fc fragment secondary antibody |
) Goat anti Rabbit IgG (FITC) |
|
43-GR59 |
Fitzgerald |
2 mg |
EUR 314 |
|
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
) Rabbit anti Goat IgG (FITC) |
|
43C-CB0523 |
Fitzgerald |
2 mg |
EUR 286 |
|
Description: Rabbit anti Goat IgG secondary antibody (FITC) |
) Rabbit anti Goat IgG (rhodamine) |
|
43C-CB0524 |
Fitzgerald |
2 mg |
EUR 291 |
|
Description: Rabbit anti Goat IgG secondary antibody (Rhodamine) |
) Rabbit anti Goat IgG (biotin) |
|
43C-CB0526 |
Fitzgerald |
2 mg |
EUR 291 |
|
Description: Rabbit anti Goat IgG secondary antibody (biotin) |
) Rabbit anti Goat IgG (HRP) |
|
43C-CB0527 |
Fitzgerald |
2 mg |
EUR 330 |
|
Description: Rabbit anti Goat IgG secondary antibody (HRP) |
Rabbit anti Goat IgG (FITC) |
|
43C-CB0530 |
Fitzgerald |
2 mg |
EUR 291 |
|
Description: Rabbit anti Goat IgG secondary antibody (FITC) |
Rabbit anti Goat IgG (rhodamine) |
|
43C-CB0531 |
Fitzgerald |
2 mg |
EUR 291 |
|
Description: Rabbit anti Goat IgG secondary antibody (Rhodamine) |
Rabbit anti Goat IgG (biotin) |
|
43C-CB0533 |
Fitzgerald |
2 mg |
EUR 301 |
|
Description: Rabbit anti Goat IgG secondary antibody (biotin) |
Rabbit anti Goat IgG (HRP) |
|
43C-CB0534 |
Fitzgerald |
2 mg |
EUR 350 |
|
Description: Rabbit anti Goat IgG secondary antibody (HRP) |
Rabbit anti Goat IgG (FITC) |
|
43C-CB0537 |
Fitzgerald |
1 mg |
EUR 311 |
|
Description: Rabbit anti Goat IgG secondary antibody (FITC) |
Rabbit anti Goat IgG (rhodamine) |
|
43C-CB0538 |
Fitzgerald |
1 mg |
EUR 284 |
|
Description: Rabbit anti Goat IgG secondary antibody (Rhodamine) |
Rabbit anti Goat IgG (biotin) |
|
43C-CB0540 |
Fitzgerald |
1 mg |
EUR 377 |
|
Description: Rabbit anti Goat IgG secondary antibody (biotin) |
Rabbit anti Goat IgG (HRP) |
|
43C-CB0541 |
Fitzgerald |
1 mg |
EUR 375 |
|
Description: Rabbit anti Goat IgG secondary antibody (HRP) |
Goat anti Rabbit IgG (FITC) |
|
43C-CB1137 |
Fitzgerald |
2 mg |
EUR 274 |
|
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
) Goat anti Rabbit IgG (rhodamine) |
|
43C-CB1138 |
Fitzgerald |
2 mg |
EUR 280 |
|
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
) Goat anti Rabbit IgG (biotin) |
|
43C-CB1140 |
Fitzgerald |
2 mg |
EUR 316 |
|
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
) Goat anti Rabbit IgG (HRP) |
|
43C-CB1141 |
Fitzgerald |
2 mg |
EUR 373 |
|
Description: Goat anti Rabbit IgG secondary antibody (HRP) |
Goat anti Rabbit IgG (FITC) |
|
43C-CB1144 |
Fitzgerald |
2 mg |
EUR 313 |
|
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
Goat anti Rabbit IgG (rhodamine) |
|
43C-CB1145 |
Fitzgerald |
2 mg |
EUR 330 |
|
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
Goat anti Rabbit IgG (biotin) |
|
43C-CB1147 |
Fitzgerald |
2 mg |
EUR 395 |
|
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
Goat anti Rabbit IgG (HRP) |
|
43C-CB1148 |
Fitzgerald |
2 mg |
EUR 358 |
|
Description: Goat anti Rabbit IgG secondary antibody (HRP) |
ELISA outcomes additionally confirmed that RF and ACPA titers confirmed considerably optimistic correlation with each citrullinated collagen and filaggrin OD values in sera of RA sufferers. 12G1 difficult aggravated the severity of murine arthritis. In abstract, mAb 12G1 can immediately detect citrullinated proteins in RA goal tissue and in sera of RA sufferers and 12G1 confirmed direct arthritogenic potential in vivo. This, 12G1 is perhaps helpful for analysis of RA together with seronegative RA and should assist to elucidate the pathophysiological position of citrullination in RA.
