Preferential Light-Chain Labeling of Native Monoclonal Antibodies Improves the Properties of Fluorophore Conjugates

Preferential Light-Chain Labeling of Native Monoclonal Antibodies Improves the Properties of Fluorophore Conjugates

Posted on by Cody

Site particular labeling strategies have important potential to boost the properties of antibody conjugates. While studied extensively in the context of antibody-drug conjugates (ADCs), few research have examined the influence of homogenous labeling on the properties of antibody-fluorophore conjugates (AFCs). We report the utility of pentafluorophenyl (PFP) esters, which had beforehand been proven to be fairly selective for Ok188 of the kappa gentle chain of human IGG antibodies, towards producing AFCs. We present that easy substitute of N-hydroxy succinimide (NHS) with PFP dramatically will increase the light-chain specificity of near-infrared (NIR) AFCs. Comparing the properties of AFCs labeled utilizing NHS and PFP-activated esters reveals that the latter displays lowered aggregation and improved brightness, each in vitro and in vivo.

Overall, the use of PFP esters gives a remarkably easy strategy to offer selectively labeled antibodies with improved properties. Fully automated characterization of monoclonal antibody (mAb) cost variants utilizing four-dimensional liquid chromatography-mass spectrometry (4D-LC-MS) is reported and illustrated. Charge variants resolved by cation-exchange chromatography (CEX) utilizing a salt- or pH-gradient are collected in loops put in on a a number of heart-cutting valve and consequently subjected to on-line desalting, denaturation, discount and trypsin digestion previous to LC-MS primarily based peptide mapping.

This innovation which considerably reduces turnaround time, pattern manipulation, loss and artefacts and will increase data gathering, is described in nice technical element, and utilized to characterize the cost heterogeneity related to three therapeutic mAbs. Sequence coverages > 95% are obtained for main and minor cost variants (> 1.0%). Post-translational modifications (PTMs) and modification websites are readily revealed in a repeatable method together with unstable succinimide intermediates which aren’t maintained when performing classical in-solution in a single day digestion of offline collected CEX peaks.

A chondroitin sulfate proteoglycan 4‑particular monoclonal antibody inhibits melanoma cell invasion in a spheroid mannequin

The overexpression of chondroitin sulfate proteoglycan 4 (CSPG4) is related to a number of tumor sorts, together with malignant melanoma, squamous cell carcinoma, triple‑unfavorable breast carcinoma, oligodendrocytomas or gliomas. Due to its restricted distribution in regular tissues, CSPG4 has been thought-about a possible goal for a number of antitumor approaches, together with monoclonal antibody (mAb) therapies. The intention of the current examine was to characterize the influence of the CSPG4‑particular mAb clone 9.2.27 by itself or together with the generally used BRAF‑selective inhibitor, PLX4032, on completely different capabilities of melanoma cells to evaluate the potential synergistic results.

The BRAF V600‑mutant human melanoma cell strains, M14 (CSPG4‑unfavorable) and WM164 (CSPG4‑optimistic), have been uncovered to the CSPG4‑particular 9.2.27 mAb and/or PLX4032. Cell viability and colony formation capability have been evaluated. A 3D‑cell tradition spheroid mannequin was used to evaluate the invasive properties of the handled cells. In addition, stream cytometric evaluation of apoptosis and cell cycle analyses have been carried out. Incubation of the WM164 cell line with CSPG4‑particular 9.2.27 mAb decreased viability, colony formation skill and the invasive capability of CSPG4‑optimistic tumor cells, which was not the case for the CSPG4‑unfavorable M14 cell line. Combined therapy of the WM164 cells with 9.2.27 mAb plus PLX4032 didn’t exert any important further impact compared to therapy with PLX4032 alone in the clonogenic and invasion assays.

Preferential Light-Chain Labeling of Native Monoclonal Antibodies Improves the Properties of Fluorophore Conjugates

M14 cell cycle distribution was not influenced by the CSPG4‑particular 9.2.27 mAb. By distinction, the publicity of WM164 cells to the mAb resulted in an arrest of the cells in the S section. Moreover, mixed therapy of the WM164 cells led to a considerably elevated accumulation of cells in the subG1 section, mixed with a lower of cells in the G2/M section. On the entire, findings of the current examine point out that the CSPG4‑particular 9.2.27 mAb exerts an anti‑invasive impact on CSPG4‑optimistic melanoma spheroids, which isn’t enhanced by BRAF inhibition. These findings present the foundation for additional investigations on the results of anti‑CSPG4‑primarily based therapies of CSPG4‑optimistic tumors.

Pathogenic Role of Circulating Citrullinated Antigens and Anti-Cyclic Monoclonal Citrullinated Peptide Antibodies in Rheumatoid Arthritis

We examined whether or not it’s potential to immediately detect citrullinated antigens in the serum of rheumatoid arthritis (RA) sufferers utilizing a monoclonal antibody (mAb) designed to be particular for citrullinated peptides. In order to verify the potential of the mAb as a direct arthritis-inducing substance by experimental mannequin of RA, a monoclonal antibody (mAb) 12G1 was generated utilizing by immunization of mice with a difficult cyclic citrullinated peptide. Immunohistochemical evaluation of RA-affected synovial tissue confirmed that our mAb 12G1 may certainly detect citrullinated proteins in goal tissues.

Subsequently, serum ranges of citrullinated sort II collagen and filaggrin have been measured in wholesome volunteers, sufferers with RA, ankylosing spondylitis (AS), and systemic lupus erythematosus (SLE) utilizing a 12G1-based sandwich ELISA. This confirmed that citrullinated filaggrin confirmed 78.9% sensitivity and 85.9% specificity for RA analysis with a cutoff optical density (OD) worth of 1.013, comparable with the outcomes from a second-generation anti-citrullinated protein antibody (ACPA) check. Circulating citrullinated collagen and filaggrin have been detected even in sera of RA sufferers who have been unfavorable for each rheumatoid issue (RF) and ACPA.

Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated
S0005 200ul
EUR 376

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)
43R-ID005AF 500 ug
EUR 338
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated
Ab8011-001 1mg
EUR 334

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated
S0013 200ul
EUR 304

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated
S0018 200ul
EUR 304

Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)
43R-ID012AF 300 ug
EUR 410
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)
43R-ID022AF 500 ug
EUR 364
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)
43R-ID047AF 500 ug
EUR 462
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated
S0014 200ul
EUR 304

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated
S0017 200ul
EUR 304

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)
43R-IR016AF 1 mg
EUR 281
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)
50337 0.5 ml
EUR 225

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)
43R-ID028AF 500 ug
EUR 430
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated
Ab8032-001 0.5mg
EUR 435

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)
43R-ID056AF 500 ug
EUR 343
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594)

Goat anti Mouse IgG1 (Alexa Fluor 488)
43R-1649 500 ug
EUR 570
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]
23158 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]
16892 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

DyLight®594 Conjugated Goat Anti-rabbit IgG (H+L)
BA1142-0.5 0.5ml
EUR 202

DyLight®594 Conjugated Goat Anti-rabbit IgG (H+L)
BA1142-1 1ml
EUR 334

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)
50336 0.5 ml
EUR 225

Anti-RPSA Alexa Fluor® 488
A4-829-C100 0.1 mg
EUR 310

Anti-CD40 antibody (Alexa-fluor 488)
STJ170000 100 µg
EUR 393
Description: CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40L (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40, predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin

Anti-LAMP3 antibody (Alexa-fluor 488)
STJ170004 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 546)
STJ170005 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 647)
STJ170006 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 488)
STJ170009 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 546)
STJ170010 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 647)
STJ170011 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 488)
STJ170014 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 546)
STJ170015 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 647)
STJ170016 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 488)
STJ170020 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 546)
STJ170021 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 647)
STJ170022 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Endoglin/CD105 Alexa Fluor
FC15024 100 Tests
EUR 448

Anti-Hu CD16 Alexa Fluor® 488
A4-646-T100 100 tests
EUR 269

EGFR antibody (Alexa Fluor 488)
61R-E109BAF 125 ug
EUR 706
Description: Mouse monoclonal EGFR antibody (Alexa Fluor 488)

SAM FCM (Alexa Fluor 647)
abx098902-100tests 100 tests
EUR 1233
  • Shipped within 5-10 working days.

SAM FCM (Alexa Fluor 488)
abx098904-60tests 60 tests
EUR 1358
  • Shipped within 5-10 working days.

iFluor™ 594 goat anti-rabbit IgG (H+L)
16628 200 ug
EUR 115
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

iFluor™ 594 goat anti-rabbit IgG (H+L)
16806 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Rabbit Anti-Goat IgG (H+L) Antibody (DyLight 594)
20-abx007407
  • EUR 272.00
  • EUR 453.00
  • 100 ul
  • 500 ul
  • Shipped within 5-10 working days.

Goat Anti-Rabbit IgG (H+L) Antibody (DyLight 594)
20-abx007433
  • EUR 272.00
  • EUR 453.00
  • 100 ul
  • 500 ul
  • Shipped within 5-10 working days.

DyLight®594 Conjugated Goat Anti-mouse IgG (H+L)
BA1141-0.5 0.5ml
EUR 202

DyLight®594 Conjugated Goat Anti-mouse IgG (H+L)
BA1141-1 1ml
EUR 334

Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated)
E0L3012-1 500ul
EUR 128

Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated)
E0L3012-2 1000ul
EUR 180

Rabbit anti Goat IgG
20-S5801R000-VO 10 ml
EUR 133
Description: Rabbit anti Goat IgG antibody

Rabbit anti Goat IgG
40C-CB0536 1.5 mg
EUR 269
Description: Rabbit anti Goat IgG secondary antibody

Goat anti Rabbit IgG
40C-CB1157 5 mg
EUR 311
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rabbit IgG
41-GR30 10 mg
EUR 277
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rabbit IgG
41-GR31 5 mg
EUR 162
Description: Goat anti Rabbit IgG secondary antibody

Rabbit anti Goat IgG
41C-CB0529 2 mg
EUR 244
Description: Rabbit anti Goat IgG secondary antibody

Goat anti Rabbit IgG
41C-CB1143 2 mg
EUR 278
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rabbit IgG
41C-CB1150 10 mg
EUR 388
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rabbit IgG
41R-1079 2 mg
EUR 149
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rabbit IgG
40-GR30 100 ml
EUR 241
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rabbit IgG
40-GR30S 100 ml
EUR 133
Description: Goat anti Rabbit IgG secondary antibody

Rabbit anti Goat IgG
E61I01002 1mg
EUR 343

goat Anti Rabbit IgG
E61I01301 1mg
EUR 274

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)
43R-ID060AF 300 ug
EUR 425
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647)

Anti-Hu CD72 Alexa Fluor® 488
A4-310-T100 100 tests
EUR 269

Anti-Bov CD9 Alexa Fluor® 488
A4-354-C100 0.1 mg
EUR 269

Anti-Hu CD30 Alexa Fluor® 700
A7-455-T100 100 tests
EUR 269

Anti-Hu CD94 Alexa Fluor® 700
A7-727-T100 100 tests
EUR 269

Anti-Hu CD56 Alexa Fluor® 700
A7-789-T100 100 tests
EUR 269

iFluor™ 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
16698 200 ug
EUR 132
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

iFluor™ 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
16833 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 349

Goat anti Rabbit IgG (H + L) (Agarose Conjugated)
43R-IG045AS 20 mg
EUR 475
Description: Goat anti Rabbit IgG (H + L) secondary antibody (Agarose Conjugated)

Rabbit anti Goat IgG (H + L) (Agarose Conjugated)
43R-IR004AS 20 mg
EUR 541
Description: Rabbit anti Goat IgG (H + L) secondary antibody (Agarose Conjugated)

HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain
AS063 100 ul
EUR 152

Goat anti- Rabbit IgG(H+L), Biotin Conjugated
E16SPB102-100 100Tests
EUR 343

Goat anti- Rabbit IgG(H+L), Biotin Conjugated
E16SPB102-1000 1000Tests
EUR 245

Goat anti- Rabbit IgG(H+L), Biotin Conjugated
E16SPB102-500 500Tests
EUR 167

Goat anti-Rabbit IgG(H+L)， FITC Conjugated
E16SPF102-050 50Tests
EUR 239

Goat anti-Rabbit IgG(H+L)， FITC Conjugated
E16SPF102-100 100Tests
EUR 343

Goat anti-Rabbit IgG(H+L)， FITC Conjugated
E16SPF102-100U 100μg
EUR 239

Goat anti-Rabbit IgG(H+L)， FITC Conjugated
E16SPF102-500U 500μg
EUR 343

Goat anti-Rabbit IgG(H+L), HRPO Conjugated
E16SPH102-100 100μl
EUR 239

Goat anti-Rabbit IgG(H+L), HRPO Conjugated
E16SPH102-1000 1000μl
EUR 434

Goat anti-Rabbit IgG(H+L), HRPO Conjugated
E16SPH102-500 500μl
EUR 343

Goat anti-Rabbit IgG(H+L)， PE Conjugated
E16SPP102-050 50Tests
EUR 239

Goat anti-Rabbit IgG(H+L)， PE Conjugated
E16SPP102-100 100Tests
EUR 343

Goat anti-Rabbit IgG(H+L)， PE Conjugated
E16SPP102-100U 100ug
EUR 239

Goat anti-Rabbit IgG(H+L)， PE Conjugated
E16SPP102-500U 500ug
EUR 343

Goat Anti-Rabbit IgG Secondary Antibody Cy3 Conjugated
L30111 1.0 ml
EUR 112

Goat Anti-Rabbit IgG Secondary Antibody FITC Conjugated
L30113 1.0 ml
EUR 97

Goat Anti-Rabbit IgG Secondary Antibody APC Conjugated
L30116 0.5 ml
EUR 191

Goat Anti-Rabbit IgG Secondary Antibody PE Conjugated
L30117 1.0 ml
EUR 191

Goat Anti-Rabbit IgG Secondary Antibody PerCP Conjugated
L30118 0.5 ml
EUR 191

Goat Anti-Rabbit IgG Secondary Antibody HRP Conjugated
L3012 1.0 ml
EUR 112

Goat Anti-Rabbit IgG Secondary Antibody Biotin Conjugated
L3014 1.0 ml
EUR 104

Goat Anti-Rabbit IgG Secondary Antibody AF488 Conjugated
L3016 1.0 ml
EUR 112

Goat Anti-Rabbit IgG Secondary Antibody AF594 Conjugated
L3017 1.0 ml
EUR 112

Goat Anti-Rabbit IgG Secondary Antibody AF647 Conjugated
L3018 1.0 ml
EUR 112

Rabbit anti-Goat IgG Secondary Antibody HRP conjugated
L3042 1.0 ml
EUR 112

Goat Anti-Rabbit IgG Secondary Antibody AF680 Conjugated
L3049 0.5 ml
EUR 126

Goat Anti-Rabbit IgG Secondary Antibody AF790 Conjugated
L30510 0.5 ml
EUR 126

Goat Anti-Rabbit IgG Secondary Antibody AMCA Conjugated
L3065 1.0 ml
EUR 97

Goat Anti-Rabbit IgG Secondary Antibody AP Conjugated
L3073 1.0 ml
EUR 119

Goat Anti-Rabbit IgG(H+L) FITC–conjugated
S0008 200ul
EUR 376

Goat Anti-Rabbit IgG(H+L) CY3–conjugated
S0011 200ul
EUR 304

Goat Anti-Rabbit IgG(H+L) TRITC–conjugated
S0015 200ul
EUR 304

Alpha Fluor™ 594 C5 Maleimide
1891 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Streptavidin-Alexa594 (Alexas fluor 594) conjugate
SV-A594-100 100 tests
EUR 225

iFluor™ 594 goat anti-mouse IgG (H+L)
16468 200 ug
EUR 115
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

iFluor™ 594 goat anti-mouse IgG (H+L)
16741 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Goat Anti-Rat IgG (H+L) Antibody (DyLight 594)
20-abx007419
  • EUR 272.00
  • EUR 453.00
  • 100 ul
  • 500 ul
  • Shipped within 5-10 working days.

Goat Anti-Mouse IgG (H+L) Antibody (DyLight 594)
20-abx007449
  • EUR 272.00
  • EUR 453.00
  • 100 ul
  • 500 ul
  • Shipped within 5-10 working days.

Goat anti Rabbit IgG Fc
41C-CB1136 2 mg
EUR 249
Description: Goat anti Rabbit IgG secondary antibody

Rabbit anti Goat IgG Fc
41R-1013 1 mg
EUR 188
Description: Rabbit anti Goat IgG Fc fragment secondary antibody

Rabbit anti Goat IgG Fc
41R-1014 1 mg
EUR 207
Description: Rabbit anti Goat IgG Fc fragment secondary antibody

Goat anti Rabbit IgG (FITC)
43-GR59 2 mg
EUR 314
Description: Goat anti Rabbit IgG secondary antibody (FITC)

Rabbit anti Goat IgG (FITC)
43C-CB0523 2 mg
EUR 286
Description: Rabbit anti Goat IgG secondary antibody (FITC)

Rabbit anti Goat IgG (rhodamine)
43C-CB0524 2 mg
EUR 291
Description: Rabbit anti Goat IgG secondary antibody (Rhodamine)

Rabbit anti Goat IgG (biotin)
43C-CB0526 2 mg
EUR 291
Description: Rabbit anti Goat IgG secondary antibody (biotin)

Rabbit anti Goat IgG (HRP)
43C-CB0527 2 mg
EUR 330
Description: Rabbit anti Goat IgG secondary antibody (HRP)

Rabbit anti Goat IgG (FITC)
43C-CB0530 2 mg
EUR 291
Description: Rabbit anti Goat IgG secondary antibody (FITC)

Rabbit anti Goat IgG (rhodamine)
43C-CB0531 2 mg
EUR 291
Description: Rabbit anti Goat IgG secondary antibody (Rhodamine)

Rabbit anti Goat IgG (biotin)
43C-CB0533 2 mg
EUR 301
Description: Rabbit anti Goat IgG secondary antibody (biotin)

Rabbit anti Goat IgG (HRP)
43C-CB0534 2 mg
EUR 350
Description: Rabbit anti Goat IgG secondary antibody (HRP)

Rabbit anti Goat IgG (FITC)
43C-CB0537 1 mg
EUR 311
Description: Rabbit anti Goat IgG secondary antibody (FITC)

Rabbit anti Goat IgG (rhodamine)
43C-CB0538 1 mg
EUR 284
Description: Rabbit anti Goat IgG secondary antibody (Rhodamine)

Rabbit anti Goat IgG (biotin)
43C-CB0540 1 mg
EUR 377
Description: Rabbit anti Goat IgG secondary antibody (biotin)

Rabbit anti Goat IgG (HRP)
43C-CB0541 1 mg
EUR 375
Description: Rabbit anti Goat IgG secondary antibody (HRP)

Goat anti Rabbit IgG (FITC)
43C-CB1137 2 mg
EUR 274
Description: Goat anti Rabbit IgG secondary antibody (FITC)

Goat anti Rabbit IgG (rhodamine)
43C-CB1138 2 mg
EUR 280
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine)

Goat anti Rabbit IgG (biotin)
43C-CB1140 2 mg
EUR 316
Description: Goat anti Rabbit IgG secondary antibody (biotin)

Goat anti Rabbit IgG (HRP)
43C-CB1141 2 mg
EUR 373
Description: Goat anti Rabbit IgG secondary antibody (HRP)

Goat anti Rabbit IgG (FITC)
43C-CB1144 2 mg
EUR 313
Description: Goat anti Rabbit IgG secondary antibody (FITC)

Goat anti Rabbit IgG (rhodamine)
43C-CB1145 2 mg
EUR 330
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine)

ELISA outcomes additionally confirmed that RF and ACPA titers confirmed considerably optimistic correlation with each citrullinated collagen and filaggrin OD values in sera of RA sufferers. 12G1 difficult aggravated the severity of murine arthritis. In abstract, mAb 12G1 can immediately detect citrullinated proteins in RA goal tissue and in sera of RA sufferers and 12G1 confirmed direct arthritogenic potential in vivo. This, 12G1 is perhaps helpful for analysis of RA together with seronegative RA and should assist to elucidate the pathophysiological position of citrullination in RA.

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