Site particular labeling strategies have important potential to boost the properties of antibody conjugates. While studied extensively in the context of antibody-drug conjugates (ADCs), few research have examined the influence of homogenous labeling on the properties of antibody-fluorophore conjugates (AFCs). We report the utility of pentafluorophenyl (PFP) esters, which had beforehand been proven to be fairly selective for Ok188 of the kappa gentle chain of human IGG antibodies, towards producing AFCs. We present that easy substitute of N-hydroxy succinimide (NHS) with PFP dramatically will increase the light-chain specificity of near-infrared (NIR) AFCs. Comparing the properties of AFCs labeled utilizing NHS and PFP-activated esters reveals that the latter displays lowered aggregation and improved brightness, each in vitro and in vivo.
Overall, the use of PFP esters gives a remarkably easy strategy to offer selectively labeled antibodies with improved properties. Fully automated characterization of monoclonal antibody (mAb) cost variants utilizing four-dimensional liquid chromatography-mass spectrometry (4D-LC-MS) is reported and illustrated. Charge variants resolved by cation-exchange chromatography (CEX) utilizing a salt- or pH-gradient are collected in loops put in on a a number of heart-cutting valve and consequently subjected to on-line desalting, denaturation, discount and trypsin digestion previous to LC-MS primarily based peptide mapping.
This innovation which considerably reduces turnaround time, pattern manipulation, loss and artefacts and will increase data gathering, is described in nice technical element, and utilized to characterize the cost heterogeneity related to three therapeutic mAbs. Sequence coverages > 95% are obtained for main and minor cost variants (> 1.0%). Post-translational modifications (PTMs) and modification websites are readily revealed in a repeatable method together with unstable succinimide intermediates which aren’t maintained when performing classical in-solution in a single day digestion of offline collected CEX peaks.
A chondroitin sulfate proteoglycan 4‑particular monoclonal antibody inhibits melanoma cell invasion in a spheroid mannequin
The overexpression of chondroitin sulfate proteoglycan 4 (CSPG4) is related to a number of tumor sorts, together with malignant melanoma, squamous cell carcinoma, triple‑unfavorable breast carcinoma, oligodendrocytomas or gliomas. Due to its restricted distribution in regular tissues, CSPG4 has been thought-about a possible goal for a number of antitumor approaches, together with monoclonal antibody (mAb) therapies. The intention of the current examine was to characterize the influence of the CSPG4‑particular mAb clone 9.2.27 by itself or together with the generally used BRAF‑selective inhibitor, PLX4032, on completely different capabilities of melanoma cells to evaluate the potential synergistic results.
The BRAF V600‑mutant human melanoma cell strains, M14 (CSPG4‑unfavorable) and WM164 (CSPG4‑optimistic), have been uncovered to the CSPG4‑particular 9.2.27 mAb and/or PLX4032. Cell viability and colony formation capability have been evaluated. A 3D‑cell tradition spheroid mannequin was used to evaluate the invasive properties of the handled cells. In addition, stream cytometric evaluation of apoptosis and cell cycle analyses have been carried out. Incubation of the WM164 cell line with CSPG4‑particular 9.2.27 mAb decreased viability, colony formation skill and the invasive capability of CSPG4‑optimistic tumor cells, which was not the case for the CSPG4‑unfavorable M14 cell line. Combined therapy of the WM164 cells with 9.2.27 mAb plus PLX4032 didn’t exert any important further impact compared to therapy with PLX4032 alone in the clonogenic and invasion assays.
M14 cell cycle distribution was not influenced by the CSPG4‑particular 9.2.27 mAb. By distinction, the publicity of WM164 cells to the mAb resulted in an arrest of the cells in the S section. Moreover, mixed therapy of the WM164 cells led to a considerably elevated accumulation of cells in the subG1 section, mixed with a lower of cells in the G2/M section. On the entire, findings of the current examine point out that the CSPG4‑particular 9.2.27 mAb exerts an anti‑invasive impact on CSPG4‑optimistic melanoma spheroids, which isn’t enhanced by BRAF inhibition. These findings present the foundation for additional investigations on the results of anti‑CSPG4‑primarily based therapies of CSPG4‑optimistic tumors.
Pathogenic Role of Circulating Citrullinated Antigens and Anti-Cyclic Monoclonal Citrullinated Peptide Antibodies in Rheumatoid Arthritis
We examined whether or not it’s potential to immediately detect citrullinated antigens in the serum of rheumatoid arthritis (RA) sufferers utilizing a monoclonal antibody (mAb) designed to be particular for citrullinated peptides. In order to verify the potential of the mAb as a direct arthritis-inducing substance by experimental mannequin of RA, a monoclonal antibody (mAb) 12G1 was generated utilizing by immunization of mice with a difficult cyclic citrullinated peptide. Immunohistochemical evaluation of RA-affected synovial tissue confirmed that our mAb 12G1 may certainly detect citrullinated proteins in goal tissues.
Subsequently, serum ranges of citrullinated sort II collagen and filaggrin have been measured in wholesome volunteers, sufferers with RA, ankylosing spondylitis (AS), and systemic lupus erythematosus (SLE) utilizing a 12G1-based sandwich ELISA. This confirmed that citrullinated filaggrin confirmed 78.9% sensitivity and 85.9% specificity for RA analysis with a cutoff optical density (OD) worth of 1.013, comparable with the outcomes from a second-generation anti-citrullinated protein antibody (ACPA) check. Circulating citrullinated collagen and filaggrin have been detected even in sera of RA sufferers who have been unfavorable for each rheumatoid issue (RF) and ACPA.
, Alexa Fluor® 488 Conjugated) Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated |
|
Ab8032-001 |
GenDepot |
0.5mg |
EUR 522 |
-Alexa 488 Fluor conjugate (adsorbed with human IgG)) Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG) |
|
50336 |
Alpha Diagnostics |
0.5 ml |
EUR 270 |
 (Alexa Fluor 594)) Donkey anti Goat IgG (H + L) (Alexa Fluor 594) |
|
43R-ID005AF |
Fitzgerald |
500 ug |
EUR 405.6 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594) |
, Biotin Conjugated) Goat anti- Rabbit IgG(H+L), Biotin Conjugated |
|
E16SPB102-100 |
EnoGene |
100Tests |
EUR 411.6 |
Goat anti- Rabbit IgG(H+L), Biotin Conjugated |
|
E16SPB102-1000 |
EnoGene |
1000Tests |
EUR 294 |
Goat anti- Rabbit IgG(H+L), Biotin Conjugated |
|
E16SPB102-500 |
EnoGene |
500Tests |
EUR 200.4 |
 (Agarose Conjugated)) Goat anti Rabbit IgG (H + L) (Agarose Conjugated) |
|
43R-IG045AS |
Fitzgerald |
20 mg |
EUR 570 |
|
Description: Goat anti Rabbit IgG (H + L) secondary antibody (Agarose Conjugated) |
 (Agarose Conjugated)) Rabbit anti Goat IgG (H + L) (Agarose Conjugated) |
|
43R-IR004AS |
Fitzgerald |
20 mg |
EUR 649.2 |
|
Description: Rabbit anti Goat IgG (H + L) secondary antibody (Agarose Conjugated) |
 (Fab 2) (Alexa Fluor 594)) Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594) |
|
43R-ID012AF |
Fitzgerald |
300 ug |
EUR 492 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594) |
) DyLight®594 Conjugated Goat Anti-rabbit IgG (H+L) |
|
BA1142-0.5 |
BosterBio |
0.5ml |
EUR 242.4 |
DyLight®594 Conjugated Goat Anti-rabbit IgG (H+L) |
|
BA1142-1 |
BosterBio |
1ml |
EUR 400.8 |
 (Alexa Fluor 647)) Donkey anti Goat IgG (H + L) (Alexa Fluor 647) |
|
43R-ID028AF |
Fitzgerald |
500 ug |
EUR 516 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647) |
 (Alexa Fluor 594)) Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594) |
|
43R-IR016AF |
Fitzgerald |
1 mg |
EUR 337.2 |
|
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594) |
) Goat anti Mouse IgG1 (Alexa Fluor 488) |
|
43R-1649 |
Fitzgerald |
500 ug |
EUR 684 |
|
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488) |
) DyLight®594 Conjugated Goat Anti-mouse IgG (H+L) |
|
BA1141-0.5 |
BosterBio |
0.5ml |
EUR 242.4 |
DyLight®594 Conjugated Goat Anti-mouse IgG (H+L) |
|
BA1141-1 |
BosterBio |
1ml |
EUR 400.8 |
 Rabbit anti-Goat IgG Secondary Antibody HRP conjugated |
|
L3042 |
SAB |
1.0 ml |
EUR 134.4 |
， PE Conjugated) Goat anti-Rabbit IgG(H+L)， PE Conjugated |
|
E16SPP102-050 |
EnoGene |
50Tests |
EUR 286.8 |
Goat anti-Rabbit IgG(H+L)， PE Conjugated |
|
E16SPP102-100 |
EnoGene |
100Tests |
EUR 411.6 |
Goat anti-Rabbit IgG(H+L)， PE Conjugated |
|
E16SPP102-100U |
EnoGene |
100ug |
EUR 286.8 |
Goat anti-Rabbit IgG(H+L)， PE Conjugated |
|
E16SPP102-500U |
EnoGene |
500ug |
EUR 411.6 |
， FITC Conjugated) Goat anti-Rabbit IgG(H+L)， FITC Conjugated |
|
E16SPF102-050 |
EnoGene |
50Tests |
EUR 286.8 |
Goat anti-Rabbit IgG(H+L)， FITC Conjugated |
|
E16SPF102-100 |
EnoGene |
100Tests |
EUR 411.6 |
Goat anti-Rabbit IgG(H+L)， FITC Conjugated |
|
E16SPF102-100U |
EnoGene |
100μg |
EUR 286.8 |
Goat anti-Rabbit IgG(H+L)， FITC Conjugated |
|
E16SPF102-500U |
EnoGene |
500μg |
EUR 411.6 |
, HRPO Conjugated) Goat anti-Rabbit IgG(H+L), HRPO Conjugated |
|
E16SPH102-100 |
EnoGene |
100μl |
EUR 286.8 |
Goat anti-Rabbit IgG(H+L), HRPO Conjugated |
|
E16SPH102-1000 |
EnoGene |
1000μl |
EUR 520.8 |
Goat anti-Rabbit IgG(H+L), HRPO Conjugated |
|
E16SPH102-500 |
EnoGene |
500μl |
EUR 411.6 |
 *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*) XFD594 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594* |
|
16404 |
AAT Bioquest |
1 mg |
EUR 166 |
 (Alexa Fluor 594)) Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
|
43R-ID022AF |
Fitzgerald |
500 ug |
EUR 436.8 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
|
43R-ID047AF |
Fitzgerald |
500 ug |
EUR 554.4 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
-Rhodamine conjugated, aff pure) Rabbit Anti-Goat IgG (Fc)-Rhodamine conjugated, aff pure |
|
30338 |
Alpha Diagnostics |
0.5 ml |
EUR 242.4 |
-Biotin conjugated, aff pure) Rabbit Anti-Goat IgG (H+L)-Biotin conjugated, aff pure |
|
30240 |
Alpha Diagnostics |
0.5 ml |
EUR 242.4 |
 Annexin V, XFD594 conjugate *XFD594 Same Structure to Alexa Fluor™ 594* |
|
20096 |
AAT Bioquest |
100 tests |
EUR 222 |
 XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594* |
|
16892 |
AAT Bioquest |
1 mg |
EUR 166 |
-Rhodamine conjugated, aff pure) Rabbit Anti-Goat IgG (H+L)-Rhodamine conjugated, aff pure |
|
30228 |
Alpha Diagnostics |
0.5 ml |
EUR 242.4 |
, Cross absorbed against human and rabbit immunoglobulinsHRPO ConjugatedHRPO Conjugated) Goat anti-Mouse IgG(H+L), Cross absorbed against human and rabbit immunoglobulinsHRPO ConjugatedHRPO Conjugated |
|
E16SPH1012-100 |
EnoGene |
100μl |
EUR 286.8 |
Goat anti-Mouse IgG(H+L), Cross absorbed against human and rabbit immunoglobulinsHRPO ConjugatedHRPO Conjugated |
|
E16SPH1012-1000 |
EnoGene |
1000μl |
EUR 520.8 |
Goat anti-Mouse IgG(H+L), Cross absorbed against human and rabbit immunoglobulinsHRPO ConjugatedHRPO Conjugated |
|
E16SPH1012-500 |
EnoGene |
500μl |
EUR 411.6 |
 Goat Anti-Rabbit IgG Secondary Antibody PE Conjugated |
|
L30117 |
SAB |
1.0 ml |
EUR 229.2 |
 Goat Anti-Rabbit IgG Secondary Antibody AP Conjugated |
|
L3073 |
SAB |
1.0 ml |
EUR 142.8 |
 Goat Anti-Rabbit IgG Secondary Antibody Cy3 Conjugated |
|
L30111 |
SAB |
1.0 ml |
EUR 134.4 |
 Goat Anti-Rabbit IgG Secondary Antibody APC Conjugated |
|
L30116 |
SAB |
0.5 ml |
EUR 229.2 |
 Goat Anti-Rabbit IgG Secondary Antibody HRP Conjugated |
|
L3012 |
SAB |
1.0 ml |
EUR 134.4 |
 secondary antibody) Cy3 Conjugated Rabbit Anti-Goat IgG (H+L) secondary antibody |
|
BA1034 |
BosterBio |
0.5ml |
EUR 218.4 |
 secondary antibody) HRP Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody |
|
BA1060-0.5 |
BosterBio |
0.5ml |
EUR 175.2 |
HRP Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody |
|
BA1060-1 |
BosterBio |
1ml |
EUR 279.6 |
 Goat Anti-Rabbit IgG Secondary Antibody FITC Conjugated |
|
L30113 |
SAB |
1.0 ml |
EUR 116.4 |
 Goat Anti-Rabbit IgG Secondary Antibody AMCA Conjugated |
|
L3065 |
SAB |
1.0 ml |
EUR 116.4 |
) Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated) |
|
E0L3012-1 |
EnoGene |
500ul |
EUR 153.6 |
Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated) |
|
E0L3012-2 |
EnoGene |
1000ul |
EUR 216 |
 secondary antibody) FITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody |
|
BA1110-0.5 |
BosterBio |
0.5mL |
EUR 157.2 |
FITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody |
|
BA1110-1 |
BosterBio |
1mL |
EUR 242.4 |
 Goat Anti-Rabbit IgG Secondary Antibody PerCP Conjugated |
|
L30118 |
SAB |
0.5 ml |
EUR 229.2 |
 Goat Anti-Rabbit IgG Secondary Antibody AF488 Conjugated |
|
L3016 |
SAB |
1.0 ml |
EUR 134.4 |
 Goat Anti-Rabbit IgG Secondary Antibody AF594 Conjugated |
|
L3017 |
SAB |
1.0 ml |
EUR 134.4 |
 Goat Anti-Rabbit IgG Secondary Antibody AF647 Conjugated |
|
L3018 |
SAB |
1.0 ml |
EUR 134.4 |
 Goat Anti-Rabbit IgG Secondary Antibody AF680 Conjugated |
|
L3049 |
SAB |
0.5 ml |
EUR 151.2 |
 Goat Anti-Rabbit IgG Secondary Antibody AF790 Conjugated |
|
L30510 |
SAB |
0.5 ml |
EUR 151.2 |
 (Agarose Conjugated)) Rabbit anti Human IgG (Fc) (Agarose Conjugated) |
|
43R-IR002AS |
Fitzgerald |
20 mg |
EUR 799.2 |
|
Description: Rabbit anti Human IgG secondary antibody (Fc) (Agarose Conjugated) |
 secondary antibody) TRITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody |
|
BA1091-0.5 |
BosterBio |
0.5ml |
EUR 224.4 |
TRITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody |
|
BA1091-1 |
BosterBio |
1ml |
EUR 400.8 |
 Goat Anti-Rabbit IgG Secondary Antibody Biotin Conjugated |
|
L3014 |
SAB |
1.0 ml |
EUR 124.8 |
 secondary antibody) Biotin Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody |
|
BA1006-0.5 |
BosterBio |
0.5ml |
EUR 115.2 |
Biotin Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody |
|
BA1006-1 |
BosterBio |
1ml |
EUR 157.2 |
) DyLight®488 Conjugated Goat Anti-rabbit IgG (H+L) |
|
BA1127-0.5 |
BosterBio |
0.5ml |
EUR 242.4 |
DyLight®488 Conjugated Goat Anti-rabbit IgG (H+L) |
|
BA1127-1 |
BosterBio |
1ml |
EUR 400.8 |
 HRPO Conjugated) Rabbit anti-Rat IgG(H+L) HRPO Conjugated |
|
E16SPH201-100 |
EnoGene |
100μl |
EUR 286.8 |
Rabbit anti-Rat IgG(H+L) HRPO Conjugated |
|
E16SPH201-1000 |
EnoGene |
1000μl |
EUR 520.8 |
Rabbit anti-Rat IgG(H+L) HRPO Conjugated |
|
E16SPH201-500 |
EnoGene |
500μl |
EUR 411.6 |
 colloidal gold conjugated (40 nm, OD10)) Rabbit Anti-Goat IgG (Fc) colloidal gold conjugated (40 nm, OD10) |
|
CG-30319 |
Alpha Diagnostics |
500 ul |
EUR 270 |
 HRP Conjugated anti-Mouse/Rabbit IgG SABC Kit |
|
SA1020 |
BosterBio |
1 kit |
EUR 206.4 |
HRP Conjugated anti-Mouse/Rabbit IgG SABC Kit |
|
SA2010 |
BosterBio |
1 kit |
EUR 426 |
 *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*) XFD594 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594* |
|
16388 |
AAT Bioquest |
1 mg |
EUR 166 |
 conjugate) Streptavidin-Alexa594 (Alexas fluor 594) conjugate |
|
SV-A594-100 |
Alpha Diagnostics |
100 tests |
EUR 270 |
 (Agarose Conjugated)) Goat anti Human IgG (Fc) (Agarose Conjugated) |
|
43R-IG016AS |
Fitzgerald |
2 ml |
EUR 457.2 |
|
Description: Goat anti Human IgG secondary antibody (Fc) (Agarose Conjugated) |
-Rhodamine (TRITC) conjugated, aff pure) Rabbit Anti-Goat IgG (H+L)-Rhodamine (TRITC) conjugated, aff pure |
|
30228-200 |
Alpha Diagnostics |
0.5 ml |
EUR 196.8 |
 colloidal gold conjugated (40 nm, OD10)) Rabbit anti-Goat IgG (H+L) colloidal gold conjugated (40 nm, OD10) |
|
CG-30219 |
Alpha Diagnostics |
500 ul |
EUR 270 |
， PE Conjugated) Goat anti-Mouse IgG(H+L)， PE Conjugated |
|
E16SPP101-020 |
EnoGene |
20Tests |
EUR 286.8 |
Goat anti-Mouse IgG(H+L)， PE Conjugated |
|
E16SPP101-050 |
EnoGene |
50Tests |
EUR 286.8 |
Goat anti-Mouse IgG(H+L)， PE Conjugated |
|
E16SPP101-100U |
EnoGene |
100ug |
EUR 286.8 |
Goat anti-Mouse IgG(H+L)， PE Conjugated |
|
E16SPP101-500U |
EnoGene |
500ug |
EUR 411.6 |
 ,Biotin Conjugated) Goat anti- Human IgG(H+L) ,Biotin Conjugated |
|
E16SPB103-100 |
EnoGene |
100Tests |
EUR 411.6 |
Goat anti- Human IgG(H+L) ,Biotin Conjugated |
|
E16SPB103-1000 |
EnoGene |
1000Tests |
EUR 294 |
Goat anti- Human IgG(H+L) ,Biotin Conjugated |
|
E16SPB103-500 |
EnoGene |
500Tests |
EUR 200.4 |
， FITC Conjugated) Goat anti-Mouse IgG(H+L)， FITC Conjugated |
|
E16SPF101-050 |
EnoGene |
50Tests |
EUR 286.8 |
Goat anti-Mouse IgG(H+L)， FITC Conjugated |
|
E16SPF101-100 |
EnoGene |
100Tests |
EUR 411.6 |
Goat anti-Mouse IgG(H+L)， FITC Conjugated |
|
E16SPF101-100U |
EnoGene |
100μg |
EUR 286.8 |
Goat anti-Mouse IgG(H+L)， FITC Conjugated |
|
E16SPF101-500U |
EnoGene |
500μg |
EUR 411.6 |
 ,HRPO Conjugated) Goat anti-Mouse IgG(H+L) ,HRPO Conjugated |
|
E16SPH101-100 |
EnoGene |
100μl |
EUR 286.8 |
Goat anti-Mouse IgG(H+L) ,HRPO Conjugated |
|
E16SPH101-1000 |
EnoGene |
1000μl |
EUR 520.8 |
Goat anti-Mouse IgG(H+L) ,HRPO Conjugated |
|
E16SPH101-500 |
EnoGene |
500μl |
EUR 411.6 |
 ,HRPO Conjugated) Goat anti-Human IgG(H+L) ,HRPO Conjugated |
|
E16SPH103-100 |
EnoGene |
100μl |
EUR 286.8 |
Goat anti-Human IgG(H+L) ,HRPO Conjugated |
|
E16SPH103-1000 |
EnoGene |
1000μl |
EUR 520.8 |
Goat anti-Human IgG(H+L) ,HRPO Conjugated |
|
E16SPH103-500 |
EnoGene |
500μl |
EUR 411.6 |
 FITC -conjugated) Goat Anti-Mouse IgG (H+L) FITC -conjugated |
|
S0007 |
Affbiotech |
200ul |
EUR 540 |
 Conjugated) Goat Anti-Rabbit IgG Secondary Antibody Rhodamine (RRX) Conjugated |
|
L30115 |
SAB |
1.0 ml |
EUR 116.4 |
 *Cross Adsorbed, XFD594 Same Structure to Alexa Fluorâ„¢ 594*) XFD594 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
|
16404-1mg |
AAT Bioquest |
1 mg |
EUR 166 |
|
|
|
Description: XFD594 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher). |
 secondary antibody) Cy3 Conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody |
|
BA1032 |
BosterBio |
0.5ml |
EUR 218.4 |
 secondary antibody) HRP Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody |
|
BA1054-0.5 |
BosterBio |
0.5ml |
EUR 175.2 |
HRP Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody |
|
BA1054-1 |
BosterBio |
1ml |
EUR 279.6 |
 (Agarose Conjugated)) Goat anti Mouse IgG (H + L) (Agarose Conjugated) |
|
43R-IG044AS |
Fitzgerald |
20 mg |
EUR 585.6 |
|
Description: Goat anti Mouse IgG (H + L) secondary antibody (Agarose Conjugated) |
 secondary antibody) FITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody |
|
BA1105-0.5 |
BosterBio |
0.5mL |
EUR 157.2 |
FITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody |
|
BA1105-1 |
BosterBio |
1mL |
EUR 242.4 |
 Conjugated) Goat Anti-Rabbit IgG Secondary Antibody Rhodamine (TRITC) Conjugated |
|
L30114 |
SAB |
1.0 ml |
EUR 116.4 |
 (Agarose Conjugated)) Goat anti Bovine IgG (H + L) (Agarose Conjugated) |
|
43R-IG043AS |
Fitzgerald |
20 mg |
EUR 478.8 |
|
Description: Goat anti Bovine IgG (H + L) secondary antibody (Agarose Conjugated) |
， Biotin Conjugated) Goat anti-Mouse IgG(H+L)， Biotin Conjugated |
|
E16SPB101-100 |
EnoGene |
100Tests |
EUR 411.6 |
Goat anti-Mouse IgG(H+L)， Biotin Conjugated |
|
E16SPB101-1000 |
EnoGene |
1000Tests |
EUR 294 |
Goat anti-Mouse IgG(H+L)， Biotin Conjugated |
|
E16SPB101-500 |
EnoGene |
500Tests |
EUR 200.4 |
 Rabbit IgG κ Light Chain Conjugated Antibody |
|
C48059 |
SAB |
100ul |
EUR 476.4 |
 secondary antibody) TRITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody |
|
BA1090-0.5 |
BosterBio |
0.5ml |
EUR 224.4 |
TRITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody |
|
BA1090-1 |
BosterBio |
1ml |
EUR 400.8 |
 secondary antibody) Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody |
|
BA1003-0.5 |
BosterBio |
0.5ml |
EUR 115.2 |
Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody |
|
BA1003-1 |
BosterBio |
1ml |
EUR 157.2 |
 colloidal gold conjugated (40 nm, OD10)) Goat anti-rabbit IgG (Fc) colloidal gold conjugated (40 nm, OD10) |
|
CG-20119 |
Alpha Diagnostics |
500 ul |
EUR 270 |
-FITC conjugate) Rabbit Anti-Goat IgG (H+L)-FITC conjugate |
|
30227-200 |
Alpha Diagnostics |
0.5 ml |
EUR 196.8 |
 *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*) XFD350 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350* |
|
16395 |
AAT Bioquest |
1 mg |
EUR 166 |
 *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*) XFD488 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488* |
|
16398 |
AAT Bioquest |
1 mg |
EUR 166 |
-HRP Conjugated Antibody) Mouse anti Rabbit IgG heavy chain(Fc)-HRP Conjugated Antibody |
|
C48069 |
SAB |
100ul |
EUR 476.4 |
 Goat Anti-Human IgG Secondary Antibody PE Conjugated |
|
L30217 |
SAB |
1.0 ml |
EUR 229.2 |
 Goat Anti-Human IgG Secondary Antibody AP Conjugated |
|
L3023 |
SAB |
1.0 ml |
EUR 142.8 |
 Goat Anti-Mouse IgG Secondary Antibody PE Conjugated |
|
L30317 |
SAB |
1.0 ml |
EUR 229.2 |
ELISA outcomes additionally confirmed that RF and ACPA titers confirmed considerably optimistic correlation with each citrullinated collagen and filaggrin OD values in sera of RA sufferers. 12G1 difficult aggravated the severity of murine arthritis. In abstract, mAb 12G1 can immediately detect citrullinated proteins in RA goal tissue and in sera of RA sufferers and 12G1 confirmed direct arthritogenic potential in vivo. This, 12G1 is perhaps helpful for analysis of RA together with seronegative RA and should assist to elucidate the pathophysiological position of citrullination in RA.
