Site particular labeling strategies have important potential to boost the properties of antibody conjugates. While studied extensively in the context of antibody-drug conjugates (ADCs), few research have examined the influence of homogenous labeling on the properties of antibody-fluorophore conjugates (AFCs). We report the utility of pentafluorophenyl (PFP) esters, which had beforehand been proven to be fairly selective for Ok188 of the kappa gentle chain of human IGG antibodies, towards producing AFCs. We present that easy substitute of N-hydroxy succinimide (NHS) with PFP dramatically will increase the light-chain specificity of near-infrared (NIR) AFCs. Comparing the properties of AFCs labeled utilizing NHS and PFP-activated esters reveals that the latter displays lowered aggregation and improved brightness, each in vitro and in vivo.
Overall, the use of PFP esters gives a remarkably easy strategy to offer selectively labeled antibodies with improved properties. Fully automated characterization of monoclonal antibody (mAb) cost variants utilizing four-dimensional liquid chromatography-mass spectrometry (4D-LC-MS) is reported and illustrated. Charge variants resolved by cation-exchange chromatography (CEX) utilizing a salt- or pH-gradient are collected in loops put in on a a number of heart-cutting valve and consequently subjected to on-line desalting, denaturation, discount and trypsin digestion previous to LC-MS primarily based peptide mapping.
This innovation which considerably reduces turnaround time, pattern manipulation, loss and artefacts and will increase data gathering, is described in nice technical element, and utilized to characterize the cost heterogeneity related to three therapeutic mAbs. Sequence coverages > 95% are obtained for main and minor cost variants (> 1.0%). Post-translational modifications (PTMs) and modification websites are readily revealed in a repeatable method together with unstable succinimide intermediates which aren’t maintained when performing classical in-solution in a single day digestion of offline collected CEX peaks.
A chondroitin sulfate proteoglycan 4‑particular monoclonal antibody inhibits melanoma cell invasion in a spheroid mannequin
The overexpression of chondroitin sulfate proteoglycan 4 (CSPG4) is related to a number of tumor sorts, together with malignant melanoma, squamous cell carcinoma, triple‑unfavorable breast carcinoma, oligodendrocytomas or gliomas. Due to its restricted distribution in regular tissues, CSPG4 has been thought-about a possible goal for a number of antitumor approaches, together with monoclonal antibody (mAb) therapies. The intention of the current examine was to characterize the influence of the CSPG4‑particular mAb clone 9.2.27 by itself or together with the generally used BRAF‑selective inhibitor, PLX4032, on completely different capabilities of melanoma cells to evaluate the potential synergistic results.
The BRAF V600‑mutant human melanoma cell strains, M14 (CSPG4‑unfavorable) and WM164 (CSPG4‑optimistic), have been uncovered to the CSPG4‑particular 9.2.27 mAb and/or PLX4032. Cell viability and colony formation capability have been evaluated. A 3D‑cell tradition spheroid mannequin was used to evaluate the invasive properties of the handled cells. In addition, stream cytometric evaluation of apoptosis and cell cycle analyses have been carried out. Incubation of the WM164 cell line with CSPG4‑particular 9.2.27 mAb decreased viability, colony formation skill and the invasive capability of CSPG4‑optimistic tumor cells, which was not the case for the CSPG4‑unfavorable M14 cell line. Combined therapy of the WM164 cells with 9.2.27 mAb plus PLX4032 didn’t exert any important further impact compared to therapy with PLX4032 alone in the clonogenic and invasion assays.

M14 cell cycle distribution was not influenced by the CSPG4‑particular 9.2.27 mAb. By distinction, the publicity of WM164 cells to the mAb resulted in an arrest of the cells in the S section. Moreover, mixed therapy of the WM164 cells led to a considerably elevated accumulation of cells in the subG1 section, mixed with a lower of cells in the G2/M section. On the entire, findings of the current examine point out that the CSPG4‑particular 9.2.27 mAb exerts an anti‑invasive impact on CSPG4‑optimistic melanoma spheroids, which isn’t enhanced by BRAF inhibition. These findings present the foundation for additional investigations on the results of anti‑CSPG4‑primarily based therapies of CSPG4‑optimistic tumors.
Pathogenic Role of Circulating Citrullinated Antigens and Anti-Cyclic Monoclonal Citrullinated Peptide Antibodies in Rheumatoid Arthritis
We examined whether or not it’s potential to immediately detect citrullinated antigens in the serum of rheumatoid arthritis (RA) sufferers utilizing a monoclonal antibody (mAb) designed to be particular for citrullinated peptides. In order to verify the potential of the mAb as a direct arthritis-inducing substance by experimental mannequin of RA, a monoclonal antibody (mAb) 12G1 was generated utilizing by immunization of mice with a difficult cyclic citrullinated peptide. Immunohistochemical evaluation of RA-affected synovial tissue confirmed that our mAb 12G1 may certainly detect citrullinated proteins in goal tissues.
Subsequently, serum ranges of citrullinated sort II collagen and filaggrin have been measured in wholesome volunteers, sufferers with RA, ankylosing spondylitis (AS), and systemic lupus erythematosus (SLE) utilizing a 12G1-based sandwich ELISA. This confirmed that citrullinated filaggrin confirmed 78.9% sensitivity and 85.9% specificity for RA analysis with a cutoff optical density (OD) worth of 1.013, comparable with the outcomes from a second-generation anti-citrullinated protein antibody (ACPA) check. Circulating citrullinated collagen and filaggrin have been detected even in sera of RA sufferers who have been unfavorable for each rheumatoid issue (RF) and ACPA.
Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594) |
43R-ID012AF |
Fitzgerald |
300 ug |
EUR 492 |
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594) |
Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
43R-ID022AF |
Fitzgerald |
500 ug |
EUR 436.8 |
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
43R-ID047AF |
Fitzgerald |
500 ug |
EUR 554.4 |
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG) |
50337 |
Alpha Diagnostics |
0.5 ml |
EUR 270 |
Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594) |
43R-IR016AF |
Fitzgerald |
1 mg |
EUR 337.2 |
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594) |
DyLight®594 Conjugated Goat Anti-rabbit IgG (H+L) |
BA1142-0.5 |
BosterBio |
0.5ml |
EUR 242.4 |
DyLight®594 Conjugated Goat Anti-rabbit IgG (H+L) |
BA1142-1 |
BosterBio |
1ml |
EUR 400.8 |
Donkey anti Goat IgG (H + L) (Alexa Fluor 647) |
43R-ID028AF |
Fitzgerald |
500 ug |
EUR 516 |
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647) |
Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated |
Ab8032-001 |
GenDepot |
0.5mg |
EUR 522 |
iFluor™ 594 goat anti-rabbit IgG (H+L) |
16628 |
AAT Bioquest |
200 ug |
EUR 138 |
|
iFluor™ 594 goat anti-rabbit IgG (H+L) |
16806 |
AAT Bioquest |
1 mg |
EUR 211.2 |
|
Rabbit Anti-Goat IgG (H+L) Antibody (DyLight 594) |
20-abx007407 |
Abbexa |
|
|
|
Goat Anti-Rabbit IgG (H+L) Antibody (DyLight 594) |
20-abx007433 |
Abbexa |
|
|
|
Donkey anti Chicken IgY (H + L) (Alexa Fluor 594) |
43R-ID056AF |
Fitzgerald |
500 ug |
EUR 411.6 |
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594) |
Goat anti Mouse IgG1 (Alexa Fluor 488) |
43R-1649 |
Fitzgerald |
500 ug |
EUR 684 |
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488) |
Rabbit anti Goat IgG |
41C-CB0529 |
Fitzgerald |
2 mg |
EUR 292.8 |
Description: Rabbit anti Goat IgG secondary antibody |
Goat anti Rabbit IgG |
41C-CB1143 |
Fitzgerald |
2 mg |
EUR 333.6 |
Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
41C-CB1150 |
Fitzgerald |
10 mg |
EUR 465.6 |
Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
41R-1079 |
Fitzgerald |
2 mg |
EUR 178.8 |
Description: Goat anti Rabbit IgG secondary antibody |
Rabbit anti Goat IgG |
40C-CB0536 |
Fitzgerald |
1.5 mg |
EUR 322.8 |
Description: Rabbit anti Goat IgG secondary antibody |
Goat anti Rabbit IgG |
40C-CB1157 |
Fitzgerald |
5 mg |
EUR 373.2 |
Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
41-GR30 |
Fitzgerald |
10 mg |
EUR 332.4 |
Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
41-GR31 |
Fitzgerald |
5 mg |
EUR 194.4 |
Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
40-GR30 |
Fitzgerald |
100 ml |
EUR 289.2 |
Description: Goat anti Rabbit IgG secondary antibody |
Goat anti Rabbit IgG |
40-GR30S |
Fitzgerald |
100 ml |
EUR 159.6 |
Description: Goat anti Rabbit IgG secondary antibody |
Rabbit anti Goat IgG |
20-S5801R000-VO |
Fitzgerald |
10 ml |
EUR 159.6 |
Description: Rabbit anti Goat IgG antibody |
Rabbit anti Goat IgG |
E61I01002 |
EnoGene |
1mg |
EUR 411.6 |
goat Anti Rabbit IgG |
E61I01301 |
EnoGene |
1mg |
EUR 328.8 |
DyLight®594 Conjugated Goat Anti-mouse IgG (H+L) |
BA1141-0.5 |
BosterBio |
0.5ml |
EUR 242.4 |
DyLight®594 Conjugated Goat Anti-mouse IgG (H+L) |
BA1141-1 |
BosterBio |
1ml |
EUR 400.8 |
Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated) |
E0L3012-1 |
EnoGene |
500ul |
EUR 153.6 |
Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated) |
E0L3012-2 |
EnoGene |
1000ul |
EUR 216 |
Rabbit Polyclonal antibody Anti-CRBN |
Anti-CRBN |
ImmunoStep |
50 µg |
EUR 418.8 |
Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG) |
50336 |
Alpha Diagnostics |
0.5 ml |
EUR 270 |
iFluor™ 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed* |
16698 |
AAT Bioquest |
200 ug |
EUR 158.4 |
|
iFluor™ 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed* |
16833 |
AAT Bioquest |
1 mg |
EUR 262.8 |
|
iFluor® 594 Styramide *Superior Replacement for Alexa Fluor 594 tyramide* |
45035 |
AAT Bioquest |
100 Slides |
EUR 334 |
Goat anti Rabbit IgG (H + L) (Agarose Conjugated) |
43R-IG045AS |
Fitzgerald |
20 mg |
EUR 570 |
Description: Goat anti Rabbit IgG (H + L) secondary antibody (Agarose Conjugated) |
Rabbit anti Goat IgG (H + L) (Agarose Conjugated) |
43R-IR004AS |
Fitzgerald |
20 mg |
EUR 649.2 |
Description: Rabbit anti Goat IgG (H + L) secondary antibody (Agarose Conjugated) |
HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain |
AS063 |
Abclonal |
100 ul |
EUR 182.4 |
Goat anti- Rabbit IgG(H+L), Biotin Conjugated |
E16SPB102-100 |
EnoGene |
100Tests |
EUR 411.6 |
Goat anti- Rabbit IgG(H+L), Biotin Conjugated |
E16SPB102-1000 |
EnoGene |
1000Tests |
EUR 294 |
Goat anti- Rabbit IgG(H+L), Biotin Conjugated |
E16SPB102-500 |
EnoGene |
500Tests |
EUR 200.4 |
Goat anti-Rabbit IgG(H+L), FITC Conjugated |
E16SPF102-050 |
EnoGene |
50Tests |
EUR 286.8 |
Goat anti-Rabbit IgG(H+L), FITC Conjugated |
E16SPF102-100 |
EnoGene |
100Tests |
EUR 411.6 |
Goat anti-Rabbit IgG(H+L), FITC Conjugated |
E16SPF102-100U |
EnoGene |
100μg |
EUR 286.8 |
Goat anti-Rabbit IgG(H+L), FITC Conjugated |
E16SPF102-500U |
EnoGene |
500μg |
EUR 411.6 |
Goat anti-Rabbit IgG(H+L), HRPO Conjugated |
E16SPH102-100 |
EnoGene |
100μl |
EUR 286.8 |
Goat anti-Rabbit IgG(H+L), HRPO Conjugated |
E16SPH102-1000 |
EnoGene |
1000μl |
EUR 520.8 |
Goat anti-Rabbit IgG(H+L), HRPO Conjugated |
E16SPH102-500 |
EnoGene |
500μl |
EUR 411.6 |
Goat anti-Rabbit IgG(H+L), PE Conjugated |
E16SPP102-050 |
EnoGene |
50Tests |
EUR 286.8 |
Goat anti-Rabbit IgG(H+L), PE Conjugated |
E16SPP102-100 |
EnoGene |
100Tests |
EUR 411.6 |
Goat anti-Rabbit IgG(H+L), PE Conjugated |
E16SPP102-100U |
EnoGene |
100ug |
EUR 286.8 |
Goat anti-Rabbit IgG(H+L), PE Conjugated |
E16SPP102-500U |
EnoGene |
500ug |
EUR 411.6 |
Goat Anti-Rabbit IgG Secondary Antibody Cy3 Conjugated |
L30111 |
SAB |
1.0 ml |
EUR 134.4 |
Goat Anti-Rabbit IgG Secondary Antibody FITC Conjugated |
L30113 |
SAB |
1.0 ml |
EUR 116.4 |
Goat Anti-Rabbit IgG Secondary Antibody APC Conjugated |
L30116 |
SAB |
0.5 ml |
EUR 229.2 |
Goat Anti-Rabbit IgG Secondary Antibody PE Conjugated |
L30117 |
SAB |
1.0 ml |
EUR 229.2 |
Goat Anti-Rabbit IgG Secondary Antibody PerCP Conjugated |
L30118 |
SAB |
0.5 ml |
EUR 229.2 |
Goat Anti-Rabbit IgG Secondary Antibody HRP Conjugated |
L3012 |
SAB |
1.0 ml |
EUR 134.4 |
Goat Anti-Rabbit IgG Secondary Antibody Biotin Conjugated |
L3014 |
SAB |
1.0 ml |
EUR 124.8 |
Goat Anti-Rabbit IgG Secondary Antibody AF488 Conjugated |
L3016 |
SAB |
1.0 ml |
EUR 134.4 |
Goat Anti-Rabbit IgG Secondary Antibody AF594 Conjugated |
L3017 |
SAB |
1.0 ml |
EUR 134.4 |
Goat Anti-Rabbit IgG Secondary Antibody AF647 Conjugated |
L3018 |
SAB |
1.0 ml |
EUR 134.4 |
Rabbit anti-Goat IgG Secondary Antibody HRP conjugated |
L3042 |
SAB |
1.0 ml |
EUR 134.4 |
Goat Anti-Rabbit IgG Secondary Antibody AF680 Conjugated |
L3049 |
SAB |
0.5 ml |
EUR 151.2 |
Goat Anti-Rabbit IgG Secondary Antibody AF790 Conjugated |
L30510 |
SAB |
0.5 ml |
EUR 151.2 |
Goat Anti-Rabbit IgG Secondary Antibody AMCA Conjugated |
L3065 |
SAB |
1.0 ml |
EUR 116.4 |
Goat Anti-Rabbit IgG Secondary Antibody AP Conjugated |
L3073 |
SAB |
1.0 ml |
EUR 142.8 |
Goat Anti-Rabbit IgG (H+L) Fluor594-conjugated |
S0006 |
Affbiotech |
200ul |
EUR 540 |
Goat Anti-Rabbit IgG (H+L) FITC-conjugated |
S0008 |
Affbiotech |
200ul |
EUR 540 |
Goat Anti-Rabbit IgG (H+L) CY3-conjugated |
S0011 |
Affbiotech |
200ul |
EUR 420 |
Goat Anti-Rabbit IgG (H+L) Fluor647-conjugated |
S0013 |
Affbiotech |
200ul |
EUR 420 |
Goat Anti-Rabbit IgG (H+L) TRITC-conjugated |
S0015 |
Affbiotech |
200ul |
EUR 420 |
Goat Anti-Rabbit IgG (H+L) Fluor488-conjugated |
S0018 |
Affbiotech |
200ul |
EUR 420 |
Rabbit anti Goat IgG (FITC) |
43C-CB0523 |
Fitzgerald |
2 mg |
EUR 343.2 |
Description: Rabbit anti Goat IgG secondary antibody (FITC) |
Rabbit anti Goat IgG (rhodamine) |
43C-CB0524 |
Fitzgerald |
2 mg |
EUR 349.2 |
Description: Rabbit anti Goat IgG secondary antibody (Rhodamine) |
Rabbit anti Goat IgG (biotin) |
43C-CB0526 |
Fitzgerald |
2 mg |
EUR 349.2 |
Description: Rabbit anti Goat IgG secondary antibody (biotin) |
Rabbit anti Goat IgG (HRP) |
43C-CB0527 |
Fitzgerald |
2 mg |
EUR 396 |
Description: Rabbit anti Goat IgG secondary antibody (HRP) |
Rabbit anti Goat IgG (FITC) |
43C-CB0530 |
Fitzgerald |
2 mg |
EUR 349.2 |
Description: Rabbit anti Goat IgG secondary antibody (FITC) |
Rabbit anti Goat IgG (rhodamine) |
43C-CB0531 |
Fitzgerald |
2 mg |
EUR 349.2 |
Description: Rabbit anti Goat IgG secondary antibody (Rhodamine) |
Rabbit anti Goat IgG (biotin) |
43C-CB0533 |
Fitzgerald |
2 mg |
EUR 361.2 |
Description: Rabbit anti Goat IgG secondary antibody (biotin) |
Rabbit anti Goat IgG (HRP) |
43C-CB0534 |
Fitzgerald |
2 mg |
EUR 420 |
Description: Rabbit anti Goat IgG secondary antibody (HRP) |
Rabbit anti Goat IgG (FITC) |
43C-CB0537 |
Fitzgerald |
1 mg |
EUR 373.2 |
Description: Rabbit anti Goat IgG secondary antibody (FITC) |
Rabbit anti Goat IgG (rhodamine) |
43C-CB0538 |
Fitzgerald |
1 mg |
EUR 340.8 |
Description: Rabbit anti Goat IgG secondary antibody (Rhodamine) |
Rabbit anti Goat IgG (biotin) |
43C-CB0540 |
Fitzgerald |
1 mg |
EUR 452.4 |
Description: Rabbit anti Goat IgG secondary antibody (biotin) |
Rabbit anti Goat IgG (HRP) |
43C-CB0541 |
Fitzgerald |
1 mg |
EUR 450 |
Description: Rabbit anti Goat IgG secondary antibody (HRP) |
Goat anti Rabbit IgG (FITC) |
43C-CB1137 |
Fitzgerald |
2 mg |
EUR 328.8 |
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
Goat anti Rabbit IgG (rhodamine) |
43C-CB1138 |
Fitzgerald |
2 mg |
EUR 336 |
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
Goat anti Rabbit IgG (biotin) |
43C-CB1140 |
Fitzgerald |
2 mg |
EUR 379.2 |
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
Goat anti Rabbit IgG (HRP) |
43C-CB1141 |
Fitzgerald |
2 mg |
EUR 447.6 |
Description: Goat anti Rabbit IgG secondary antibody (HRP) |
Goat anti Rabbit IgG (FITC) |
43C-CB1144 |
Fitzgerald |
2 mg |
EUR 375.6 |
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
Goat anti Rabbit IgG (rhodamine) |
43C-CB1145 |
Fitzgerald |
2 mg |
EUR 396 |
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
Goat anti Rabbit IgG (biotin) |
43C-CB1147 |
Fitzgerald |
2 mg |
EUR 474 |
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
Goat anti Rabbit IgG (HRP) |
43C-CB1148 |
Fitzgerald |
2 mg |
EUR 429.6 |
Description: Goat anti Rabbit IgG secondary antibody (HRP) |
Goat anti Rabbit IgG (rhodamine) |
43C-CB1152 |
Fitzgerald |
2 mg |
EUR 346.8 |
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
Goat anti Rabbit IgG (biotin) |
43C-CB1154 |
Fitzgerald |
2 mg |
EUR 373.2 |
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
Goat anti Rabbit IgG (HRP) |
43C-CB1155 |
Fitzgerald |
2 mg |
EUR 442.8 |
Description: Goat anti Rabbit IgG secondary antibody (HRP) |
Goat anti Rabbit IgG (FITC) |
43C-CB1158 |
Fitzgerald |
2 mg |
EUR 465.6 |
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
Goat anti Rabbit IgG (rhodamine) |
43C-CB1159 |
Fitzgerald |
2 mg |
EUR 465.6 |
Description: Goat anti Rabbit IgG secondary antibody (Rhodamine) |
Goat anti Rabbit IgG (biotin) |
43C-CB1161 |
Fitzgerald |
2 mg |
EUR 478.8 |
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
Goat anti Rabbit IgG (HRP) |
43C-CB1162 |
Fitzgerald |
2 mg |
EUR 483.6 |
Description: Goat anti Rabbit IgG secondary antibody (HRP) |
Goat anti Rabbit IgG (biotin) |
43R-1493 |
Fitzgerald |
2 mg |
EUR 219.6 |
Description: Goat anti Rabbit IgG secondary antibody (biotin) |
Goat anti Rabbit IgG (FITC) |
43R-1494 |
Fitzgerald |
2 mg |
EUR 232.8 |
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
Goat anti Rabbit IgG (HRP) |
43R-1495 |
Fitzgerald |
1 mg |
EUR 165.6 |
Description: Goat anti Rabbit IgG secondary antibody (HRP) |
Goat anti Rabbit IgG (FITC) |
43-GR59 |
Fitzgerald |
2 mg |
EUR 376.8 |
Description: Goat anti Rabbit IgG secondary antibody (FITC) |
Goat anti Rabbit IgG Fc |
41C-CB1136 |
Fitzgerald |
2 mg |
EUR 298.8 |
Description: Goat anti Rabbit IgG secondary antibody |
Rabbit anti Goat IgG Fc |
41R-1013 |
Fitzgerald |
1 mg |
EUR 225.6 |
Description: Rabbit anti Goat IgG Fc fragment secondary antibody |
Rabbit anti Goat IgG Fc |
41R-1014 |
Fitzgerald |
1 mg |
EUR 248.4 |
Description: Rabbit anti Goat IgG Fc fragment secondary antibody |
Rabbit Anti-Goat IgG Antibody |
20-abx400048 |
Abbexa |
-
EUR 226.80
-
EUR 134.40
-
EUR 326.40
|
|
|
Goat Anti-Rabbit IgG Antibody |
20-abx400049 |
Abbexa |
-
EUR 226.80
-
EUR 134.40
-
EUR 326.40
|
|
|
Goat anti Rabbit IgG Fc |
70R-IG011 |
Fitzgerald |
1.5 mg |
EUR 453.6 |
Description: Goat anti Rabbit IgG Fc secondary antibody |
Goat anti Rabbit IgG Fc |
70R-IG017 |
Fitzgerald |
2 mg |
EUR 457.2 |
Description: Goat anti Rabbit IgG Fc secondary antibody |
Rabbit Anti-Sheep/Goat IgG |
C020231-100mg |
Unibiotest |
100mg |
EUR 1641.6 |
Rabbit Anti-Sheep/Goat IgG |
C020231-1mg |
Unibiotest |
1mg |
EUR 322.8 |
Goat anti-rabbit IgG-HRP |
E22NR02-1 |
EnoGene |
100ul |
EUR 411.6 |
Goat Anti-Rabbit IgG/HRP |
E3GAR001 |
EnoGene |
200ul |
EUR 489.6 |
Rabbit Anti-Goat IgG/HRPAntibody |
E3RAG001 |
EnoGene |
200ul |
EUR 489.6 |
goat Anti Rabbit IgG-Biotin |
E61I01302 |
EnoGene |
1mg |
EUR 598.8 |
goat Anti Rabbit IgG-HRP |
E61I01303 |
EnoGene |
1mg |
EUR 598.8 |
goat Anti Rabbit IgG-FITC |
E61I01304 |
EnoGene |
1mg |
EUR 733.2 |
Goat Anti-Rabbit IgG-HRP |
LF-SA8002 |
Abfrontier |
0.5 ml |
EUR 145.2 |
Description: Goat polyclonal to Rabbit IgG |
Goat Anti-Rabbit IgG-HRP |
LF-SA8002A |
Abfrontier |
1.0 ml (0.5 ml X 2) |
EUR 205.2 |
Description: Goat polyclonal to Rabbit IgG |
Goat Anti-Rabbit IgG-AP |
LF-SA8002AP |
Abfrontier |
1.0 ml |
EUR 374.4 |
Description: Goat polyclonal to Rabbit IgG |
Goat Anti-Rabbit IgG-BIOT |
LF-SA8002B |
Abfrontier |
1.0 mg |
EUR 398.4 |
Description: Goat polyclonal to Rabbit IgG |
Goat Anti-Rabbit IgG-FITC |
LF-SA8002F |
Abfrontier |
1.0 mg |
EUR 313.2 |
Description: Goat polyclonal to Rabbit IgG |
Goat Anti-Rabbit IgG-TRITC |
LF-SA8002TR |
Abfrontier |
1.0 mg |
EUR 297.6 |
Description: Goat polyclonal to Rabbit IgG |
Goat Anti-Rabbit IgG-UNLB |
LF-SA8002U |
Abfrontier |
1.0 mg |
EUR 297.6 |
Description: Goat polyclonal to Rabbit IgG |
Goat Anti-Rabbit IgG-PE |
LF-SA8060 |
Abfrontier |
1 ml |
EUR 494.4 |
Description: Goat polyclonal to Rabbit IgG |
488 Goat-anti-Rabbit IgG |
GT26001 |
Neuromics |
1 mg |
EUR 450 |
494 Goat-anti-Rabbit IgG |
GT26002 |
Neuromics |
1 mg |
EUR 450 |
546 Goat-anti-Rabbit IgG |
GT26004 |
Neuromics |
1 mg |
EUR 450 |
642 Goat-anti-Rabbit IgG |
GT26006 |
Neuromics |
1 mg |
EUR 450 |
ELISA outcomes additionally confirmed that RF and ACPA titers confirmed considerably optimistic correlation with each citrullinated collagen and filaggrin OD values in sera of RA sufferers. 12G1 difficult aggravated the severity of murine arthritis. In abstract, mAb 12G1 can immediately detect citrullinated proteins in RA goal tissue and in sera of RA sufferers and 12G1 confirmed direct arthritogenic potential in vivo. This, 12G1 is perhaps helpful for analysis of RA together with seronegative RA and should assist to elucidate the pathophysiological position of citrullination in RA.