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Preferential Light-Chain Labeling of Native Monoclonal Antibodies Improves the Properties of Fluorophore Conjugates

Preferential Light-Chain Labeling of Native Monoclonal Antibodies Improves the Properties of Fluorophore Conjugates

Posted on September 3, 2021September 4, 2021 by Cody

Site particular labeling strategies have important potential to boost the properties of antibody conjugates. While studied extensively in the context of antibody-drug conjugates (ADCs), few research have examined the influence of homogenous labeling on the properties of antibody-fluorophore conjugates (AFCs). We report the utility of pentafluorophenyl (PFP) esters, which had beforehand been proven to be fairly selective for Ok188 of the kappa gentle chain of human IGG antibodies, towards producing AFCs. We present that easy substitute of N-hydroxy succinimide (NHS) with PFP dramatically will increase the light-chain specificity of near-infrared (NIR) AFCs. Comparing the properties of AFCs labeled utilizing NHS and PFP-activated esters reveals that the latter displays lowered aggregation and improved brightness, each in vitro and in vivo.

Overall, the use of PFP esters gives a remarkably easy strategy to offer selectively labeled antibodies with improved properties. Fully automated characterization of monoclonal antibody (mAb) cost variants utilizing four-dimensional liquid chromatography-mass spectrometry (4D-LC-MS) is reported and illustrated. Charge variants resolved by cation-exchange chromatography (CEX) utilizing a salt- or pH-gradient are collected in loops put in on a a number of heart-cutting valve and consequently subjected to on-line desalting, denaturation, discount and trypsin digestion previous to LC-MS primarily based peptide mapping.

This innovation which considerably reduces turnaround time, pattern manipulation, loss and artefacts and will increase data gathering, is described in nice technical element, and utilized to characterize the cost heterogeneity related to three therapeutic mAbs. Sequence coverages > 95% are obtained for main and minor cost variants (> 1.0%). Post-translational modifications (PTMs) and modification websites are readily revealed in a repeatable method together with unstable succinimide intermediates which aren’t maintained when performing classical in-solution in a single day digestion of offline collected CEX peaks.

A chondroitin sulfate proteoglycan 4‑particular monoclonal antibody inhibits melanoma cell invasion in a spheroid mannequin

The overexpression of chondroitin sulfate proteoglycan 4 (CSPG4) is related to a number of tumor sorts, together with malignant melanoma, squamous cell carcinoma, triple‑unfavorable breast carcinoma, oligodendrocytomas or gliomas. Due to its restricted distribution in regular tissues, CSPG4 has been thought-about a possible goal for a number of antitumor approaches, together with monoclonal antibody (mAb) therapies. The intention of the current examine was to characterize the influence of the CSPG4‑particular mAb clone 9.2.27 by itself or together with the generally used BRAF‑selective inhibitor, PLX4032, on completely different capabilities of melanoma cells to evaluate the potential synergistic results.

The BRAF V600‑mutant human melanoma cell strains, M14 (CSPG4‑unfavorable) and WM164 (CSPG4‑optimistic), have been uncovered to the CSPG4‑particular 9.2.27 mAb and/or PLX4032. Cell viability and colony formation capability have been evaluated. A 3D‑cell tradition spheroid mannequin was used to evaluate the invasive properties of the handled cells. In addition, stream cytometric evaluation of apoptosis and cell cycle analyses have been carried out. Incubation of the WM164 cell line with CSPG4‑particular 9.2.27 mAb decreased viability, colony formation skill and the invasive capability of CSPG4‑optimistic tumor cells, which was not the case for the CSPG4‑unfavorable M14 cell line. Combined therapy of the WM164 cells with 9.2.27 mAb plus PLX4032 didn’t exert any important further impact compared to therapy with PLX4032 alone in the clonogenic and invasion assays.

Preferential Light-Chain Labeling of Native Monoclonal Antibodies Improves the Properties of Fluorophore Conjugates

M14 cell cycle distribution was not influenced by the CSPG4‑particular 9.2.27 mAb. By distinction, the publicity of WM164 cells to the mAb resulted in an arrest of the cells in the S section. Moreover, mixed therapy of the WM164 cells led to a considerably elevated accumulation of cells in the subG1 section, mixed with a lower of cells in the G2/M section. On the entire, findings of the current examine point out that the CSPG4‑particular 9.2.27 mAb exerts an anti‑invasive impact on CSPG4‑optimistic melanoma spheroids, which isn’t enhanced by BRAF inhibition. These findings present the foundation for additional investigations on the results of anti‑CSPG4‑primarily based therapies of CSPG4‑optimistic tumors.

Pathogenic Role of Circulating Citrullinated Antigens and Anti-Cyclic Monoclonal Citrullinated Peptide Antibodies in Rheumatoid Arthritis

We examined whether or not it’s potential to immediately detect citrullinated antigens in the serum of rheumatoid arthritis (RA) sufferers utilizing a monoclonal antibody (mAb) designed to be particular for citrullinated peptides. In order to verify the potential of the mAb as a direct arthritis-inducing substance by experimental mannequin of RA, a monoclonal antibody (mAb) 12G1 was generated utilizing by immunization of mice with a difficult cyclic citrullinated peptide. Immunohistochemical evaluation of RA-affected synovial tissue confirmed that our mAb 12G1 may certainly detect citrullinated proteins in goal tissues.

Subsequently, serum ranges of citrullinated sort II collagen and filaggrin have been measured in wholesome volunteers, sufferers with RA, ankylosing spondylitis (AS), and systemic lupus erythematosus (SLE) utilizing a 12G1-based sandwich ELISA. This confirmed that citrullinated filaggrin confirmed 78.9% sensitivity and 85.9% specificity for RA analysis with a cutoff optical density (OD) worth of 1.013, comparable with the outcomes from a second-generation anti-citrullinated protein antibody (ACPA) check. Circulating citrullinated collagen and filaggrin have been detected even in sera of RA sufferers who have been unfavorable for each rheumatoid issue (RF) and ACPA.

Goat Anti-Rabbit IgG(H+L)(Alexa Fluor 594 conjugated)

MBS2548772-02mL MyBiosource 0.2mL
EUR 125

Goat Anti-Rabbit IgG(H+L)(Alexa Fluor 594 conjugated)

MBS2548772-5x02mL MyBiosource 5x0.2mL
EUR 515

Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG (H+L)

E45G021N EnoGene 50 ul
EUR 57.25

Goat Anti-Mouse IgG(H+L)(Alexa Fluor 594 conjugated)

MBS2548771-006mL MyBiosource 0.06mL
EUR 95

Goat Anti-Mouse IgG(H+L)(Alexa Fluor 594 conjugated)

MBS2548771-012mL MyBiosource 0.12mL
EUR 105

Goat Anti-Mouse IgG(H+L)(Alexa Fluor 594 conjugated)

MBS2548771-02mL MyBiosource 0.2mL
EUR 125

Goat Anti-Mouse IgG(H+L)(Alexa Fluor 594 conjugated)

MBS2548771-5x02mL MyBiosource 5x0.2mL
EUR 515

Goat Anti-Rabbit IgG (H+L)(Alexa Fluor 488 conjugated)

MBS2548767-006mL MyBiosource 0.06mL
EUR 95

Goat Anti-Rabbit IgG (H+L)(Alexa Fluor 488 conjugated)

MBS2548767-012mL MyBiosource 0.12mL
EUR 105

Goat Anti-Rabbit IgG (H+L)(Alexa Fluor 488 conjugated)

MBS2548767-02mL MyBiosource 0.2mL
EUR 125

Goat Anti-Rabbit IgG (H+L)(Alexa Fluor 488 conjugated)

MBS2548767-5x02mL MyBiosource 5x0.2mL
EUR 515

Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate)

S0B4007-100L Starter Biotechnology Co Ltd 100 µL
EUR 78

Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate)

S0B4007-1mL Starter Biotechnology Co Ltd 1 mL
EUR 500

Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate)

S0B4007-25uL Starter Biotechnology Co Ltd 25 μL
EUR 20

Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate)

S0B4007-each Starter Biotechnology Co Ltd each Ask for price

Alexa Fluor 405-conjugated Goat Anti-Rabbit IgG (H+L)

E45G016N EnoGene 50 ul
EUR 57.25

Goat Anti-Mouse IgG (H+L)(Alexa Fluor 488 conjugated)

MBS2548768-006mL MyBiosource 0.06mL
EUR 95

Goat Anti-Mouse IgG (H+L)(Alexa Fluor 488 conjugated)

MBS2548768-012mL MyBiosource 0.12mL
EUR 105

Goat Anti-Mouse IgG (H+L)(Alexa Fluor 488 conjugated)

MBS2548768-02mL MyBiosource 0.2mL
EUR 125

Goat Anti-Mouse IgG (H+L)(Alexa Fluor 488 conjugated)

MBS2548768-5x02mL MyBiosource 5x0.2mL
EUR 515

Goat anti-Mouse IgG(H+L) (Alexa Fluor® 594 Conjugate)

S0B4010-100uL Starter Biotechnology Co Ltd 100 μL
EUR 70

Goat anti-Mouse IgG(H+L) (Alexa Fluor® 594 Conjugate)

S0B4010-1mL Starter Biotechnology Co Ltd 1 mL
EUR 500

Goat anti-Mouse IgG(H+L) (Alexa Fluor® 594 Conjugate)

S0B4010-25uL Starter Biotechnology Co Ltd 25 μL
EUR 20

Goat anti-Mouse IgG(H+L) (Alexa Fluor® 594 Conjugate)

S0B4010-each Starter Biotechnology Co Ltd each Ask for price

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated

Ab8011-001 GenDepot 1mg
EUR 400.8

Alexa Fluor 405-conjugated Goat Anti-Mouse IgG (H+L)

E45G010N EnoGene 50 ul
EUR 57.25

Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate)

S0B4004-100L Starter Biotechnology Co Ltd 100 µL
EUR 78

Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate)

S0B4004-1mL Starter Biotechnology Co Ltd 1 mL
EUR 500

Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate)

S0B4004-25L Starter Biotechnology Co Ltd 25 µL
EUR 20

Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate)

S0B4004-500L Starter Biotechnology Co Ltd 500 µL
EUR 280

Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate)

S0B4005-100L Starter Biotechnology Co Ltd 100 µL
EUR 78

Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate)

S0B4005-1mL Starter Biotechnology Co Ltd 1 mL
EUR 500

Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate)

S0B4005-25L Starter Biotechnology Co Ltd 25 µL
EUR 20

Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate)

S0B4005-each Starter Biotechnology Co Ltd each Ask for price

Goat anti-Mouse IgG(H+L) (Alexa Fluor® 647 Conjugate)

S0B4008-100L Starter Biotechnology Co Ltd 100 µL
EUR 78

Goat anti-Mouse IgG(H+L) (Alexa Fluor® 647 Conjugate)

S0B4008-1mL Starter Biotechnology Co Ltd 1 mL
EUR 500

Goat anti-Mouse IgG(H+L) (Alexa Fluor® 647 Conjugate)

S0B4008-25uL Starter Biotechnology Co Ltd 25 μL
EUR 20

Goat anti-Mouse IgG(H+L) (Alexa Fluor® 647 Conjugate)

S0B4008-each Starter Biotechnology Co Ltd each Ask for price

Lipopolysaccharide, Alexa Fluor 594 conjugated

LPS-05AF Creative BioMart 100µg
EUR 615.2

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated

Ab8032-001 GenDepot 0.5mg
EUR 522

Rabbit Anti-Goat IgG (H&L) - Alexa Fluor 594

DL87372A-100ul DL Develop 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Goat IgG (H&L) - Alexa Fluor 594

DL87372A-500ul DL Develop 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Goat IgG (H&L) - Alexa Fluor 594

E38A3618 EnoGene 100μL
EUR 125

Rabbit Anti-Goat IgG (H&L) - Alexa Fluor 594

YLF0350-100ul Shanghai YL Biotech 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Goat IgG (H&L) - Alexa Fluor 594

YLF0350-1ml Shanghai YL Biotech 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Goat IgG (H&L) - Alexa Fluor 594

YLF0350-500ul Shanghai YL Biotech 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)

50336 Alpha Diagnostics 0.5 ml
EUR 270

Goat Anti-Rabbit IgG (H&L) - Alexa Fluor 594

DL87365A-100ul DL Develop 100 ul
EUR 140
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Rabbit IgG (H&L) - Alexa Fluor 594

DL87365A-500ul DL Develop 500 ul
EUR 330
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Rabbit IgG (H&L) - Alexa Fluor 594

E38A3611 EnoGene 100μL
EUR 125

Goat Anti-Rabbit IgG (H&L) - Alexa Fluor 594

YLF0111-100ul Shanghai YL Biotech 100 ul
EUR 100
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Rabbit IgG (H&L) - Alexa Fluor 594

YLF0111-1ml Shanghai YL Biotech 1 ml
EUR 490
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Rabbit IgG (H&L) - Alexa Fluor 594

YLF0111-500ul Shanghai YL Biotech 500 ul
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Rabbit IgG(H+L)(Elab Fluor®594 conjugated)

E-AB-1060-120uL Elabscience Biotech 120uL
EUR 34
Description: Elab Fluor®594

Goat Anti-Rabbit IgG(H+L)(Elab Fluor®594 conjugated)

E-AB-1060-500uL Elabscience Biotech 500uL
EUR 100
Description: Elab Fluor®594

Goat Anti-Rabbit IgG(H+L)(Elab Fluor®594 conjugated)

E-AB-1060-60uL Elabscience Biotech 60uL
EUR 21
Description: Elab Fluor®594

Goat Anti-Rabbit IgG(H+L)(Elab Fluor®594 conjugated)

E-AB-1060-each Elabscience Biotech each Ask for price
Description: Elab Fluor®594

Goat Anti-Human IgG Fc - Alexa Fluor 594

DL87358A-100ul DL Develop 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

DL87358A-500ul DL Develop 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

YLF0339-100ul Shanghai YL Biotech 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

YLF0339-1ml Shanghai YL Biotech 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

YLF0339-500ul Shanghai YL Biotech 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

DL87357A-100ul DL Develop 100 ul
EUR 190
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

DL87357A-500ul DL Develop 500 ul
EUR 280
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

E38A3603 EnoGene 100μL
EUR 125

Goat Anti-Human IgG Fab - Alexa Fluor 594

YLF0558-100ul Shanghai YL Biotech 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

YLF0558-1ml Shanghai YL Biotech 1 ml
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

YLF0558-500ul Shanghai YL Biotech 500 ul
EUR 210
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

DL87376A-100ul DL Develop 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

DL87376A-500ul DL Develop 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

E38A3622 EnoGene 100μL
EUR 125

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

YLF0354-100ul Shanghai YL Biotech 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

YLF0354-1ml Shanghai YL Biotech 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

YLF0354-500ul Shanghai YL Biotech 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Rabbit IgG F(ab')2(Elab Fluor®594 conjugated)

E-AB-1106-120uL Elabscience Biotech 120uL
EUR 34
Description: Elab Fluor®594

Goat Anti-Rabbit IgG F(ab')2(Elab Fluor®594 conjugated)

E-AB-1106-500uL Elabscience Biotech 500uL
EUR 100
Description: Elab Fluor®594

Goat Anti-Rabbit IgG F(ab')2(Elab Fluor®594 conjugated)

E-AB-1106-60uL Elabscience Biotech 60uL
EUR 21
Description: Elab Fluor®594

Goat Anti-Rabbit IgG F(ab')2(Elab Fluor®594 conjugated)

E-AB-1106-each Elabscience Biotech each Ask for price
Description: Elab Fluor®594

Goat Anti-Rabbit IgG(H+L) pAb, Alexa Fluor 594

E40SA2130 EnoGene 100ul
EUR 120

Goat Anti-Rabbit IgG(H+L) pAb, Alexa Fluor 594

E40SA2152 EnoGene 100ul
EUR 120

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87364A-100ul DL Develop 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87364A-500ul DL Develop 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

E38A3610 EnoGene 100μL
EUR 125

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0343-100ul Shanghai YL Biotech 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0343-1ml Shanghai YL Biotech 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0343-500ul Shanghai YL Biotech 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

DL87356A-100ul DL Develop 100 ul
EUR 140
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

DL87356A-500ul DL Develop 500 ul
EUR 330
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Mouse IgG (H&L) - Alexa Fluor 594

DL87362A-100ul DL Develop 100 ul
EUR 140
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Mouse IgG (H&L) - Alexa Fluor 594

DL87362A-500ul DL Develop 500 ul
EUR 330
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

E38A3602 EnoGene 100μL
EUR 125

Goat Anti-Mouse IgG (H&L) - Alexa Fluor 594

E38A3608 EnoGene 100μL
EUR 125

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

YLF0109-100ul Shanghai YL Biotech 100 ul
EUR 100
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

YLF0109-1ml Shanghai YL Biotech 1 ml
EUR 490
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

YLF0109-500ul Shanghai YL Biotech 500 ul
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Mouse IgG (H&L) - Alexa Fluor 594

YLF0110-100ul Shanghai YL Biotech 100 ul
EUR 100
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Mouse IgG (H&L) - Alexa Fluor 594

YLF0110-1ml Shanghai YL Biotech 1 ml
EUR 490
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Mouse IgG (H&L) - Alexa Fluor 594

YLF0110-500ul Shanghai YL Biotech 500 ul
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Cat IgG (H&L) - Alexa Fluor 594

DL87367A-100ul DL Develop 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Cat IgG (H&L) - Alexa Fluor 594

DL87367A-500ul DL Develop 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Dog IgG (H&L) - Alexa Fluor 594

DL87369A-100ul DL Develop 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Dog IgG (H&L) - Alexa Fluor 594

DL87369A-500ul DL Develop 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87382A-100ul DL Develop 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87382A-500ul DL Develop 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Rat IgG (H&L) - Alexa Fluor 594

DL87383A-100ul DL Develop 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Rat IgG (H&L) - Alexa Fluor 594

DL87383A-500ul DL Develop 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Cat IgG (H&L) - Alexa Fluor 594

E38A3613 EnoGene 100μL
EUR 125

Rabbit Anti-Dog IgG (H&L) - Alexa Fluor 594

E38A3615 EnoGene 100μL
EUR 125

Rabbit Anti-Pig IgG (H&L) - Alexa Fluor 594

E38A3628 EnoGene 100μL
EUR 125

Rabbit Anti-Rat IgG (H&L) - Alexa Fluor 594

E38A3629 EnoGene 100μL
EUR 125

Rabbit Anti-Cat IgG (H&L) - Alexa Fluor 594

YLF0345-100ul Shanghai YL Biotech 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Cat IgG (H&L) - Alexa Fluor 594

YLF0345-1ml Shanghai YL Biotech 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Cat IgG (H&L) - Alexa Fluor 594

YLF0345-500ul Shanghai YL Biotech 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Dog IgG (H&L) - Alexa Fluor 594

YLF0347-100ul Shanghai YL Biotech 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Dog IgG (H&L) - Alexa Fluor 594

YLF0347-1ml Shanghai YL Biotech 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Dog IgG (H&L) - Alexa Fluor 594

YLF0347-500ul Shanghai YL Biotech 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0358-100ul Shanghai YL Biotech 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0358-1ml Shanghai YL Biotech 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0358-500ul Shanghai YL Biotech 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Rat IgG (H&L) - Alexa Fluor 594

YLF0359-100ul Shanghai YL Biotech 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Rat IgG (H&L) - Alexa Fluor 594

YLF0359-1ml Shanghai YL Biotech 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Rat IgG (H&L) - Alexa Fluor 594

YLF0359-500ul Shanghai YL Biotech 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Mouse IgG(H+L)(Elab Fluor®594 conjugated)

E-AB-1059-120uL Elabscience Biotech 120uL
EUR 34
Description: Elab Fluor®594

Goat Anti-Mouse IgG(H+L)(Elab Fluor®594 conjugated)

E-AB-1059-500uL Elabscience Biotech 500uL
EUR 100
Description: Elab Fluor®594

Goat Anti-Mouse IgG(H+L)(Elab Fluor®594 conjugated)

E-AB-1059-60uL Elabscience Biotech 60uL
EUR 21
Description: Elab Fluor®594

Goat Anti-Mouse IgG(H+L)(Elab Fluor®594 conjugated)

E-AB-1059-each Elabscience Biotech each Ask for price
Description: Elab Fluor®594

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)

43R-ID005AF Fitzgerald 500 ug
EUR 333
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Rabbit Anti-Horse IgG (H&L) - Alexa Fluor 594

DL87374A-100ul DL Develop 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Horse IgG (H&L) - Alexa Fluor 594

DL87374A-500ul DL Develop 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG (H&L) - Alexa Fluor 594

DL87375A-100ul DL Develop 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG (H&L) - Alexa Fluor 594

DL87375A-500ul DL Develop 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Mouse IgG (H&L) - Alexa Fluor 594

DL87381A-100ul DL Develop 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Mouse IgG (H&L) - Alexa Fluor 594

DL87381A-500ul DL Develop 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
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ELISA outcomes additionally confirmed that RF and ACPA titers confirmed considerably optimistic correlation with each citrullinated collagen and filaggrin OD values in sera of RA sufferers. 12G1 difficult aggravated the severity of murine arthritis. In abstract, mAb 12G1 can immediately detect citrullinated proteins in RA goal tissue and in sera of RA sufferers and 12G1 confirmed direct arthritogenic potential in vivo. This, 12G1 is perhaps helpful for analysis of RA together with seronegative RA and should assist to elucidate the pathophysiological position of citrullination in RA.

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  • 4 Host (6)
  • 96T (6)
  • apoptosis (7)
  • array (6)
  • Assay (6)
  • Bacteria (7)
  • Blocking (6)
  • Combination of pembrolizumab and 125I attenuates the aggressiveness of non-small cell lung cancer. (1)
  • Comparison of Efficacy of Topical Curcumin Gel with Triamcinolone-hyaluronidase Gel Individually and in Combination in the Treatment of Oral Submucous Fibrosis. (1)
  • Comprehensive analysis of the long non-coding RNA-associated competitive endogenous RNA network reveals novel prognostic biomarkers in Wilms' tumor. (1)
  • GMO (6)
  • Goat (6)
  • Increased expression of plakoglobin is associated with upregulated MAPK and PI3K/AKT signalling pathways in early resectable pancreatic ductal adenocarcinoma. (1)
  • LINC00460 accelerates progression of ovarian cancer by activating transcriptional factor ZNF703. (1)
  • Next-generation laser-based photodynamic endoscopic diagnosis using 5-aminolevulinic acid for early gastric adenocarcinoma and gastric adenoma. (1)
  • Pig (6)
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  • Prone en-bloc sacrectomy with proctectomy: A surgical approach to the inaccessible and hostile pelvis (1)
  • Sleeve Gastrectomy: Surgical Technique, Outcomes, and Complications (34)

Categories

  • 4 Host
  • 96T
  • apoptosis
  • array
  • Assay
  • Bacteria
  • Blocking
  • Combination of pembrolizumab and 125I attenuates the aggressiveness of non-small cell lung cancer.
  • Comparison of Efficacy of Topical Curcumin Gel with Triamcinolone-hyaluronidase Gel Individually and in Combination in the Treatment of Oral Submucous Fibrosis.
  • Comprehensive analysis of the long non-coding RNA-associated competitive endogenous RNA network reveals novel prognostic biomarkers in Wilms' tumor.
  • GMO
  • Goat
  • Increased expression of plakoglobin is associated with upregulated MAPK and PI3K/AKT signalling pathways in early resectable pancreatic ductal adenocarcinoma.
  • LINC00460 accelerates progression of ovarian cancer by activating transcriptional factor ZNF703.
  • Next-generation laser-based photodynamic endoscopic diagnosis using 5-aminolevulinic acid for early gastric adenocarcinoma and gastric adenoma.
  • Pig
  • Pigeon
  • Plant
  • plasmid
  • Plate
  • Prone en-bloc sacrectomy with proctectomy: A surgical approach to the inaccessible and hostile pelvis
  • Sleeve Gastrectomy: Surgical Technique, Outcomes, and Complications
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