Aspergillus flavus is a major fungal pathogen of vegetation and an opportunistic pathogen of people. In addition to the direct affect of an infection, it produces immunosuppressive and carcinogenic aflatoxins. The early detection of A. flavus is due to this fact essential to diagnose and monitor fungal an infection, to stop aflatoxin contamination of meals and feed, and for efficient antifungal remedy. Aspergillus-specific monoclonal antibodies (mAbs) are promising as diagnostic and therapeutic reagents for the monitoring and therapy of Aspergillus infections, respectively.
However, A. flavus has a posh cell wall composition and dynamic morphology, hindering the discovery of mAbs with well-characterized targets. Here we describe the technology and detailed characterization of mAb5.52 (IgG2aκ) and mAb17.15 (IgG1κ), which bind particularly to the extremely immunogenic cell wall antigen A. flavus mannoprotein 1 (Aflmp1). Both mAbs have been generated utilizing hybridoma know-how following the immunization of mice with a recombinant truncated model of Aflmp1 (ExD, together with the homologous CR4 area) produced in micro organism. We present that mAb5.52 and mAb17.15 bind particularly to A. flavus and A. parasiticus cell wall fragments (CWFs), with no cross-reaction to CWFs from different fungal pathogens.
Immunofluorescence microscopy revealed that each mAbs bind to the floor of Aspergillus hyphae and that mAb17.15 additionally binds to spores. The epitope for each mAbs is localized inside the CR4 area of the Aflmp1 protein. These Aspergillus-specific mAbs could also be helpful for the early detection of fungal an infection in meals/feed crops, for serodiagnosis in sufferers with invasive aspergillosis attributable to A. flavus an infection and for the improvement of antibody-expressing disease-resistant crops. The epidermal development issue receptor (EGFR) is a tyrosine kinase receptor that performs an necessary function in regular epidermal cell physiology.
EGFR is overexpressed in most cancers cells and has a quantity of mutations that implicate tumor malignancy, improvement, and poor affected person prognosis; thus, EGFR is a pretty goal for most cancers remedy. At current, anti-EGFR monoclonal antibodies (mAbs) have been authorized and are used for treating sufferers with a range of EGFR-expressing cancers.
Tubulointerstitial nephritis in antineutrophil cytoplasmic antibody-associated vasculitis with monoclonal gammopathy
Isolated tubulointerstitial nephritis (TIN) with out glomerular crescent formation is a uncommon manifestation of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). Some sufferers with monoclonal gammopathy of undetermined significance current with renal issues as a result of serum monoclonal protein. Here, we current a case of TIN presumably attributable to AAV with monoclonal gammopathy. Laboratory knowledge revealed acute kidney damage, elevated C-reactive protein (CRP) and ANCA titers, and elevated tubular damage markers. Renal biopsy revealed TIN with no obvious glomerular lesion. The findings of peritubular capillaritis and tubulitis indicated that AAV had contributed to the improvement of TIN.
However, in situ hybridization for free mild chains revealed kappa mild chain restriction, indicating that the involvement of monoclonal gammopathy in the pathogenesis of TIN stays potential. The affected person additionally developed ophthalmic neuropathy, in all probability attributable to AAV. Oral prednisone (0.6 mg/kg/day) administration improved each the ocular signs and the laboratory parameters. Our case demonstrated that the concurrence of AAV and monoclonal gammopathy might pose a diagnostic dilemma in distinguishing the trigger of TIN. Besides, some studies recommend an affiliation between AAV and monoclonal gammopathy, though direct proof is missing. Further analysis is required to determine this affiliation.
CD44 capabilities as a major hyaluronan receptor on most cell varieties, with roles in cell adhesion, migration, proliferation, differentiation, and survival. The CD44 gene includes 20 exons, with different splicing producing many various isoforms. CD44 variant isoforms exhibit tissue-specific expression patterns and have been studied as therapeutic targets for a number of cancers; due to this fact, anti-CD44 monoclonal antibodies (mAbs) are helpful for investigating CD44 expression in varied cancers.

Isolation and characterization of porcine monoclonal antibodies revealed two distinct serotype-independent epitopes on VP2 of foot-and-mouth illness virus
Pigs are prone to foot-and-mouth illness virus (FMDV), and the humoral immune response performs a vital function in safety in opposition to FMDV an infection. However, little info is obtainable about FMDV-specific mAbs derived from single B cells of pigs. This research aimed to find out the antigenic options of FMDV which are acknowledged by antibodies from pigs. Therefore, a panel of pig-derived mAbs in opposition to FMDV have been developed utilizing fluorescence-based single B cell antibody know-how. Western blotting revealed that three of the antibodies acknowledged conserved antigen epitopes on capsid protein VP2, and exhibited broad reactivity in opposition to each FMDV serotypes A and O.
Recombinant Human Angiotensinogen Protein |
RP01021 |
Abclonal |
5 μg |
EUR 183 |
Rat Angiotensinogen (AGT) Protein |
20-abx065353 |
Abbexa |
-
EUR 578.00
-
EUR 258.00
-
EUR 1720.00
-
EUR 690.00
-
EUR 425.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Angiotensinogen (1-14) , Rat |
5-00699 |
CHI Scientific |
4 x 5mg |
Ask for price |
Rat Angiotensinogen (AGT) Protein |
20-abx651468 |
Abbexa |
-
EUR 662.00
-
EUR 272.00
-
EUR 2012.00
-
EUR 787.00
-
EUR 481.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Rat Angiotensinogen ELISA kit |
E02A0497-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Rat Angiotensinogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Angiotensinogen ELISA kit |
E02A0497-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Rat Angiotensinogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Angiotensinogen ELISA kit |
E02A0497-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Rat Angiotensinogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat angiotensinogen ELISA kit |
55R-2262 |
Fitzgerald |
1 kit |
EUR 1373 |
Description: Rat angiotensinogen ELISA kit for use in research |
Angiotensinogen Peptide |
20-abx266414 |
Abbexa |
-
EUR 523.00
-
EUR 871.00
-
EUR 384.00
|
|
|
Angiotensinogen Peptide |
20-abx266467 |
Abbexa |
-
EUR 551.00
-
EUR 926.00
-
EUR 398.00
|
|
|
Angiotensinogen Peptide |
20-abx265209 |
Abbexa |
-
EUR 565.00
-
EUR 954.00
-
EUR 398.00
|
|
|
Angiotensinogen Peptide |
20-abx265535 |
Abbexa |
-
EUR 551.00
-
EUR 926.00
-
EUR 398.00
|
|
|
Angiotensinogen Antibody |
49159-100ul |
SAB |
100ul |
EUR 333 |
Angiotensinogen Antibody |
49159-50ul |
SAB |
50ul |
EUR 239 |
Angiotensinogen antibody |
10R-A130a |
Fitzgerald |
200 ul |
EUR 565 |
Description: Mouse monoclonal Angiotensinogen antibody |
Angiotensinogen antibody |
10R-A131a |
Fitzgerald |
200 ul |
EUR 457 |
Description: Mouse monoclonal Angiotensinogen antibody |
Angiotensinogen antibody |
70R-12752 |
Fitzgerald |
100 ul |
EUR 457 |
Description: Affinity purified Rabbit polyclonal Angiotensinogen antibody |
Angiotensinogen antibody |
70R-13411 |
Fitzgerald |
100 ul |
EUR 457 |
Description: Affinity purified Rabbit polyclonal Angiotensinogen antibody |
Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol) |
RAT-5 |
Alpha Diagnostics |
1 |
EUR 1138 |
Rat Agt/ Angiotensinogen ELISA Kit |
E0038Ra |
Sunlong |
1 Kit |
EUR 571 |
Rat Angiotensinogen (AGT) ELISA Kit |
abx573648-96tests |
Abbexa |
96 tests |
EUR 715 |
|
Rat angiotensinogen(aGT)ELISA Kit |
GA-E0665RT-48T |
GenAsia Biotech |
48T |
EUR 373 |
Rat angiotensinogen(aGT)ELISA Kit |
GA-E0665RT-96T |
GenAsia Biotech |
96T |
EUR 609 |
Rat Angiotensinogen (AGT) CLIA Kit |
20-abx492048 |
Abbexa |
-
EUR 8569.00
-
EUR 4560.00
-
EUR 1052.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Rat Angiotensinogen (AGT) ELISA Kit |
abx256349-96tests |
Abbexa |
96 tests |
EUR 668 |
|
Rat Angiotensinogen (AGT) ELISA Kit |
20-abx155199 |
Abbexa |
-
EUR 7237.00
-
EUR 3855.00
-
EUR 895.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Rat Angiotensinogen (AGT) CLIA Kit |
abx195130-96tests |
Abbexa |
96 tests |
EUR 825 |
|
Rat Angiotensinogen (AGT) ELISA Kit |
DLR-AGT-Ra-48T |
DL Develop |
48T |
EUR 454 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Angiotensinogen (AGT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Angiotensinogen (AGT) ELISA Kit |
DLR-AGT-Ra-96T |
DL Develop |
96T |
EUR 587 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Angiotensinogen (AGT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Angiotensinogen (AGT) ELISA Kit |
RD-AGT-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 450 |
Rat Angiotensinogen (AGT) ELISA Kit |
RD-AGT-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 622 |
Rat Angiotensinogen ELISA Kit (AGT) |
RK03477 |
Abclonal |
96 Tests |
EUR 521 |
Rat Angiotensinogen (AGT) ELISA Kit |
RDR-AGT-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 470 |
Rat Angiotensinogen (AGT) ELISA Kit |
RDR-AGT-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 651 |
Rat Angiotensinogen (AGT) Protein (Active) |
20-abx655558 |
Abbexa |
-
EUR 913.00
-
EUR 328.00
-
EUR 2973.00
-
EUR 1107.00
-
EUR 634.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Rat Agt(Angiotensinogen) ELISA Kit |
ER0371 |
FN Test |
96T |
EUR 524.1 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.938 ng/ml |
Angiotensinogen (AGT) Monoclonal Antibody (Rat) |
4-MAA797Ra21 |
Cloud-Clone |
-
EUR 247.00
-
EUR 2510.00
-
EUR 625.00
-
EUR 310.00
-
EUR 214.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against Rat Angiotensinogen (AGT) |
Rat angiotensinogen (aGT) ELISA Kit |
CSB-E08565r-24T |
Cusabio |
1 plate of 24 wells |
EUR 165 |
|
Description: Quantitativesandwich ELISA kit for measuring Rat angiotensinogen (aGT) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Rat angiotensinogen (aGT) ELISA Kit |
1-CSB-E08565r |
Cusabio |
-
EUR 967.00
-
EUR 5925.00
-
EUR 3134.00
|
- 1 plate of 96 wells
- 10 plates of 96 wells each
- 5 plates of 96 wells each
|
|
Description: Quantitativesandwich ELISA kit for measuring Rat angiotensinogen (aGT) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Rat Angiotensinogen (AGT) ELISA Kit |
SEA797Ra-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 4626.78 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiotensinogen (AGT) in serum, plasma, tissue homogenates and other biological fluids. |
Rat Angiotensinogen (AGT) ELISA Kit |
SEA797Ra-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 468.68 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiotensinogen (AGT) in serum, plasma, tissue homogenates and other biological fluids. |
Rat Angiotensinogen (AGT) ELISA Kit |
SEA797Ra-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 626.68 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiotensinogen (AGT) in serum, plasma, tissue homogenates and other biological fluids. |
Rat Angiotensinogen (AGT) ELISA Kit |
SEA797Ra-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2520.06 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiotensinogen (AGT) in serum, plasma, tissue homogenates and other biological fluids. |
Rat Angiotensinogen (AGT) ELISA Kit |
4-SEA797Ra |
Cloud-Clone |
-
EUR 4677.00
-
EUR 2471.00
-
EUR 627.00
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Angiotensinogen (AGT) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. |
Angiotensinogen (AGT) Polyclonal Antibody (Rat) |
4-PAA797Ra01 |
Cloud-Clone |
-
EUR 243.00
-
EUR 2457.00
-
EUR 613.00
-
EUR 305.00
-
EUR 212.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Rat Angiotensinogen (AGT) |
Rat angiotensinogen,aGT ELISA Kit |
CN-01790R1 |
ChemNorm |
96T |
EUR 447 |
Rat angiotensinogen,aGT ELISA Kit |
CN-01790R2 |
ChemNorm |
48T |
EUR 296 |
Angiotensinogen (AGT) Antibody |
20-abx301357 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Angiotensinogen (AGT) Antibody |
20-abx128540 |
Abbexa |
-
EUR 398.00
-
EUR 133.00
-
EUR 1107.00
-
EUR 537.00
-
EUR 314.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Angiotensinogen (AGT) Antibody |
20-abx110982 |
Abbexa |
|
|
|
Angiotensinogen Rabbit mAb |
A11689-100ul |
Abclonal |
100 ul |
EUR 410 |
Angiotensinogen Rabbit mAb |
A11689-200ul |
Abclonal |
200 ul |
EUR 571 |
Angiotensinogen Rabbit mAb |
A11689-20ul |
Abclonal |
20 ul |
EUR 221 |
Angiotensinogen Rabbit mAb |
A11689-50ul |
Abclonal |
50 ul |
EUR 287 |
Angiotensinogen (AGT) Antibody |
20-abx101137 |
Abbexa |
-
EUR 398.00
-
EUR 133.00
-
EUR 1107.00
-
EUR 537.00
-
EUR 314.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Angiotensinogen (AGT) Antibody |
20-abx101138 |
Abbexa |
-
EUR 411.00
-
EUR 133.00
-
EUR 1135.00
-
EUR 551.00
-
EUR 314.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Angiotensinogen (AGT) Antibody |
20-abx101139 |
Abbexa |
-
EUR 425.00
-
EUR 133.00
-
EUR 1177.00
-
EUR 578.00
-
EUR 328.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Angiotensinogen (AGT) Antibody |
20-abx101140 |
Abbexa |
-
EUR 328.00
-
EUR 815.00
-
EUR 425.00
-
EUR 154.00
-
EUR 258.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Angiotensinogen (AGT) Antibody |
abx010360-100ul |
Abbexa |
100 ul |
EUR 411 |
|
Angiotensinogen (AGT) Antibody |
20-abx004798 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Angiotensinogen (AGT) Antibody |
abx033720-400ul |
Abbexa |
400 ul |
EUR 523 |
|
Angiotensinogen (AGT) Antibody |
abx033720-80l |
Abbexa |
80 µl |
EUR 286 |
|
Angiotensinogen (AGT) Antibody |
abx033721-400ul |
Abbexa |
400 ul |
EUR 523 |
|
Angiotensinogen (AGT) Antibody |
abx033721-80l |
Abbexa |
80 µl |
EUR 286 |
|
Angiotensinogen (AGT) Antibody |
20-abx171240 |
Abbexa |
|
|
|
Angiotensinogen (AGT) Antibody |
20-abx320213 |
Abbexa |
|
|
|
Angiotensinogen (AGT) Antibody |
20-abx322250 |
Abbexa |
|
|
|
Angiotensinogen (AGT) Antibody |
abx230393-100ug |
Abbexa |
100 ug |
EUR 509 |
|
Angiotensinogen (AGT) Antibody |
abx230394-100ug |
Abbexa |
100 ug |
EUR 481 |
|
Angiotensinogen (AGT) Antibody |
abx230395-100ug |
Abbexa |
100 ug |
EUR 481 |
|
Angiotensinogen (AGT) Antibody |
20-abx339646 |
Abbexa |
|
|
|
Angiotensinogen (AGT) Antibody |
20-abx241726 |
Abbexa |
|
|
|
Angiotensinogen (AGT) Antibody |
20-abx241946 |
Abbexa |
|
|
|
Angiotensinogen (AGT) Antibody |
20-abx241947 |
Abbexa |
|
|
|
Angiotensinogen (AGT) Antibody |
20-abx242324 |
Abbexa |
|
|
|
Angiotensinogen (AGT) Antibody |
20-abx214416 |
Abbexa |
|
|
|
Angiotensinogen (AGT) Antibody |
20-abx175400 |
Abbexa |
|
|
|
Angiotensinogen (AGT) Antibody |
20-abx211344 |
Abbexa |
|
|
|
Human Angiotensinogen Antibody |
11013-05011 |
AssayPro |
150 ug |
EUR 217 |
Angiotensinogen Conjugated Antibody |
C49159 |
SAB |
100ul |
EUR 397 |
Human Angiotensinogen (AGT) |
1-CSB-EP001463HU1 |
Cusabio |
-
EUR 380.00
-
EUR 214.00
-
EUR 1309.00
-
EUR 560.00
-
EUR 873.00
-
EUR 262.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human Angiotensinogen(AGT),partial expressed in E.coli |
Eukaryotic Angiotensinogen (AGT) |
4-EPA797Mu61 |
Cloud-Clone |
-
EUR 466.46
-
EUR 228.00
-
EUR 1474.24
-
EUR 558.08
-
EUR 1016.16
-
EUR 375.00
-
EUR 3535.60
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Mouse Angiotensinogen expressed in: 293F cell |
Eukaryotic Angiotensinogen (AGT) |
4-EPA797Ra61 |
Cloud-Clone |
-
EUR 471.84
-
EUR 229.00
-
EUR 1494.40
-
EUR 564.80
-
EUR 1029.60
-
EUR 379.00
-
EUR 3586.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Rat Angiotensinogen expressed in: 293F cell |
anti- Angiotensinogen antibody |
FNab00393 |
FN Test |
100µg |
EUR 548.75 |
|
Description: Antibody raised against Angiotensinogen |
anti- Angiotensinogen antibody |
FNab00394 |
FN Test |
100µg |
EUR 505.25 |
|
Description: Antibody raised against Angiotensinogen |
anti- Angiotensinogen antibody |
FNab00395 |
FN Test |
100µg |
EUR 505.25 |
|
Description: Antibody raised against Angiotensinogen |
ELISA kit for Rat AGT (Angiotensinogen) |
E-EL-R0199 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 534 |
|
Description: A sandwich ELISA kit for quantitative measurement of Rat AGT (Angiotensinogen) in samples from Serum, Plasma, Cell supernatant |
CLIA kit for Rat AGT (Angiotensinogen) |
E-CL-R0138 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 584 |
|
Description: A sandwich CLIA kit for quantitative measurement of Rat AGT (Angiotensinogen) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Rat AGT (Angiotensinogen) |
ELK4678 |
ELK Biotech |
1 plate of 96 wells |
EUR 432 |
|
Description: A sandwich ELISA kit for detection of Angiotensinogen from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Angiotensinogen (AGT) Monoclonal Antibody (Rat), APC |
4-MAA797Ra21-APC |
Cloud-Clone |
-
EUR 345.00
-
EUR 3275.00
-
EUR 912.00
-
EUR 440.00
-
EUR 219.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against Rat Angiotensinogen (AGT). This antibody is labeled with APC. |
Angiotensinogen (AGT) Monoclonal Antibody (Rat), Biotinylated |
4-MAA797Ra21-Biotin |
Cloud-Clone |
-
EUR 311.00
-
EUR 2460.00
-
EUR 727.00
-
EUR 381.00
-
EUR 219.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against Rat Angiotensinogen (AGT). This antibody is labeled with Biotin. |
An alanine-substitution scanning assay and sequence conservation evaluation elucidated that these porcine mAbs acknowledged two conserved epitopes on VP2: a linear epitope in the N terminus and a conformational epitope involving residues Ok63, H65, L66, F67, D68 and L81 on two β-sheets (B-sheet and C-sheet) that trusted the integrity of VP2. Random parings of heavy and mild chains of the IgGs confirmed that the heavy chain is predominantly concerned in binding to antigen. The mild chain of porcine IgG contributes to the binding affinity towards an antigen and might operate as a help platform for antibody stability. In abstract, this research is the first to disclose the conserved antigenic profile of FMDV acknowledged by porcine B cells and offers a novel technique for analysing the antibody response in opposition to FMDV in its pure hosts (i.e. pigs) at the clonal stage.