Selection and characterization of two monoclonal antibodies specific for the Aspergillus flavus major antigenic cell wall protein Aflmp1

Selection and characterization of two monoclonal antibodies specific for the Aspergillus flavus major antigenic cell wall protein Aflmp1

Posted on by Cody

Aspergillus flavus is a major fungal pathogen of vegetation and an opportunistic pathogen of people. In addition to the direct affect of an infection, it produces immunosuppressive and carcinogenic aflatoxins. The early detection of A. flavus is due to this fact essential to diagnose and monitor fungal an infection, to stop aflatoxin contamination of meals and feed, and for efficient antifungal remedy. Aspergillus-specific monoclonal antibodies (mAbs) are promising as diagnostic and therapeutic reagents for the monitoring and therapy of Aspergillus infections, respectively.

However, A. flavus has a posh cell wall composition and dynamic morphology, hindering the discovery of mAbs with well-characterized targets. Here we describe the technology and detailed characterization of mAb5.52 (IgG2aκ) and mAb17.15 (IgG1κ), which bind particularly to the extremely immunogenic cell wall antigen A. flavus mannoprotein 1 (Aflmp1). Both mAbs have been generated utilizing hybridoma know-how following the immunization of mice with a recombinant truncated model of Aflmp1 (ExD, together with the homologous CR4 area) produced in micro organism. We present that mAb5.52 and mAb17.15 bind particularly to A. flavus and A. parasiticus cell wall fragments (CWFs), with no cross-reaction to CWFs from different fungal pathogens.

Immunofluorescence microscopy revealed that each mAbs bind to the floor of Aspergillus hyphae and that mAb17.15 additionally binds to spores. The epitope for each mAbs is localized inside the CR4 area of the Aflmp1 protein. These Aspergillus-specific mAbs could also be helpful for the early detection of fungal an infection in meals/feed crops, for serodiagnosis in sufferers with invasive aspergillosis attributable to A. flavus an infection and for the improvement of antibody-expressing disease-resistant crops. The epidermal development issue receptor (EGFR) is a tyrosine kinase receptor that performs an necessary function in regular epidermal cell physiology.

EGFR is overexpressed in most cancers cells and has a quantity of mutations that implicate tumor malignancy, improvement, and poor affected person prognosis; thus, EGFR is a pretty goal for most cancers remedy. At current, anti-EGFR monoclonal antibodies (mAbs) have been authorized and are used for treating sufferers with a range of EGFR-expressing cancers.

Tubulointerstitial nephritis in antineutrophil cytoplasmic antibody-associated vasculitis with monoclonal gammopathy

Isolated tubulointerstitial nephritis (TIN) with out glomerular crescent formation is a uncommon manifestation of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). Some sufferers with monoclonal gammopathy of undetermined significance current with renal issues as a result of serum monoclonal protein. Here, we current a case of TIN presumably attributable to AAV with monoclonal gammopathy. Laboratory knowledge revealed acute kidney damage, elevated C-reactive protein (CRP) and ANCA titers, and elevated tubular damage markers. Renal biopsy revealed TIN with no obvious glomerular lesion. The findings of peritubular capillaritis and tubulitis indicated that AAV had contributed to the improvement of TIN.

However, in situ hybridization for free mild chains revealed kappa mild chain restriction, indicating that the involvement of monoclonal gammopathy in the pathogenesis of TIN stays potential. The affected person additionally developed ophthalmic neuropathy, in all probability attributable to AAV. Oral prednisone (0.6 mg/kg/day) administration improved each the ocular signs and the laboratory parameters. Our case demonstrated that the concurrence of AAV and monoclonal gammopathy might pose a diagnostic dilemma in distinguishing the trigger of TIN. Besides, some studies recommend an affiliation between AAV and monoclonal gammopathy, though direct proof is missing. Further analysis is required to determine this affiliation.

CD44 capabilities as a major hyaluronan receptor on most cell varieties, with roles in cell adhesion, migration, proliferation, differentiation, and survival. The CD44 gene includes 20 exons, with different splicing producing many various isoforms. CD44 variant isoforms exhibit tissue-specific expression patterns and have been studied as therapeutic targets for a number of cancers; due to this fact, anti-CD44 monoclonal antibodies (mAbs) are helpful for investigating CD44 expression in varied cancers.

Selection and characterization of two monoclonal antibodies specific for the Aspergillus flavus major antigenic cell wall protein Aflmp1

Isolation and characterization of porcine monoclonal antibodies revealed two distinct serotype-independent epitopes on VP2 of foot-and-mouth illness virus

Pigs are prone to foot-and-mouth illness virus (FMDV), and the humoral immune response performs a vital function in safety in opposition to FMDV an infection. However, little info is obtainable about FMDV-specific mAbs derived from single B cells of pigs. This research aimed to find out the antigenic options of FMDV which are acknowledged by antibodies from pigs. Therefore, a panel of pig-derived mAbs in opposition to FMDV have been developed utilizing fluorescence-based single B cell antibody know-how. Western blotting revealed that three of the antibodies acknowledged conserved antigen epitopes on capsid protein VP2, and exhibited broad reactivity in opposition to each FMDV serotypes A and O.

Recombinant Human Angiotensinogen Protein
RP01021 5 μg
EUR 183

Rat Angiotensinogen (AGT) Protein
20-abx065353
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiotensinogen (1-14) , Rat
5-00699 4 x 5mg Ask for price

Rat Angiotensinogen (AGT) Protein
20-abx651468
  • EUR 662.00
  • EUR 272.00
  • EUR 2012.00
  • EUR 787.00
  • EUR 481.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Angiotensinogen ELISA kit
E02A0497-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Angiotensinogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Angiotensinogen ELISA kit
E02A0497-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Angiotensinogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Angiotensinogen ELISA kit
E02A0497-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Angiotensinogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat angiotensinogen ELISA kit
55R-2262 1 kit
EUR 1373
Description: Rat angiotensinogen ELISA kit for use in research

Angiotensinogen Peptide
20-abx266414
  • EUR 523.00
  • EUR 871.00
  • EUR 384.00
  • 10 mg
  • 25 mg
  • 5 mg

Angiotensinogen Peptide
20-abx266467
  • EUR 551.00
  • EUR 926.00
  • EUR 398.00
  • 10 mg
  • 25 mg
  • 5 mg

Angiotensinogen Peptide
20-abx265209
  • EUR 565.00
  • EUR 954.00
  • EUR 398.00
  • 10 mg
  • 25 mg
  • 5 mg

Angiotensinogen Peptide
20-abx265535
  • EUR 551.00
  • EUR 926.00
  • EUR 398.00
  • 10 mg
  • 25 mg
  • 5 mg

Angiotensinogen Antibody
49159-100ul 100ul
EUR 333

Angiotensinogen Antibody
49159-50ul 50ul
EUR 239

Angiotensinogen antibody
10R-A130a 200 ul
EUR 565
Description: Mouse monoclonal Angiotensinogen antibody

Angiotensinogen antibody
10R-A131a 200 ul
EUR 457
Description: Mouse monoclonal Angiotensinogen antibody

Angiotensinogen antibody
70R-12752 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal Angiotensinogen antibody

Angiotensinogen antibody
70R-13411 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal Angiotensinogen antibody

Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)
RAT-5 1
EUR 1138

Rat Agt/ Angiotensinogen ELISA Kit
E0038Ra 1 Kit
EUR 571

Rat Angiotensinogen (AGT) ELISA Kit
abx573648-96tests 96 tests
EUR 715

Rat angiotensinogen(aGT)ELISA Kit
GA-E0665RT-48T 48T
EUR 373

Rat angiotensinogen(aGT)ELISA Kit
GA-E0665RT-96T 96T
EUR 609

Rat Angiotensinogen (AGT) CLIA Kit
20-abx492048
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Angiotensinogen (AGT) ELISA Kit
abx256349-96tests 96 tests
EUR 668

Rat Angiotensinogen (AGT) ELISA Kit
20-abx155199
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Angiotensinogen (AGT) CLIA Kit
abx195130-96tests 96 tests
EUR 825

Rat Angiotensinogen (AGT) ELISA Kit
DLR-AGT-Ra-48T 48T
EUR 454
Description: A sandwich quantitative ELISA assay kit for detection of Rat Angiotensinogen (AGT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Angiotensinogen (AGT) ELISA Kit
DLR-AGT-Ra-96T 96T
EUR 587
Description: A sandwich quantitative ELISA assay kit for detection of Rat Angiotensinogen (AGT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Angiotensinogen (AGT) ELISA Kit
RD-AGT-Ra-48Tests 48 Tests
EUR 450

Rat Angiotensinogen (AGT) ELISA Kit
RD-AGT-Ra-96Tests 96 Tests
EUR 622

Rat Angiotensinogen ELISA Kit (AGT)
RK03477 96 Tests
EUR 521

Rat Angiotensinogen (AGT) ELISA Kit
RDR-AGT-Ra-48Tests 48 Tests
EUR 470

Rat Angiotensinogen (AGT) ELISA Kit
RDR-AGT-Ra-96Tests 96 Tests
EUR 651

Rat Angiotensinogen (AGT) Protein (Active)
20-abx655558
  • EUR 913.00
  • EUR 328.00
  • EUR 2973.00
  • EUR 1107.00
  • EUR 634.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Agt(Angiotensinogen) ELISA Kit
ER0371 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.938 ng/ml

Angiotensinogen (AGT) Monoclonal Antibody (Rat)
4-MAA797Ra21
  • EUR 247.00
  • EUR 2510.00
  • EUR 625.00
  • EUR 310.00
  • EUR 214.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Rat Angiotensinogen (AGT)

Rat angiotensinogen (aGT) ELISA Kit
CSB-E08565r-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Rat angiotensinogen (aGT) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat angiotensinogen (aGT) ELISA Kit
1-CSB-E08565r
  • EUR 967.00
  • EUR 5925.00
  • EUR 3134.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat angiotensinogen (aGT) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Angiotensinogen (AGT) ELISA Kit
SEA797Ra-10x96wellstestplate 10x96-wells test plate
EUR 4626.78
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiotensinogen (AGT) in serum, plasma, tissue homogenates and other biological fluids.

Rat Angiotensinogen (AGT) ELISA Kit
SEA797Ra-1x48wellstestplate 1x48-wells test plate
EUR 468.68
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiotensinogen (AGT) in serum, plasma, tissue homogenates and other biological fluids.

Rat Angiotensinogen (AGT) ELISA Kit
SEA797Ra-1x96wellstestplate 1x96-wells test plate
EUR 626.68
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiotensinogen (AGT) in serum, plasma, tissue homogenates and other biological fluids.

Rat Angiotensinogen (AGT) ELISA Kit
SEA797Ra-5x96wellstestplate 5x96-wells test plate
EUR 2520.06
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Angiotensinogen (AGT) in serum, plasma, tissue homogenates and other biological fluids.

Rat Angiotensinogen (AGT) ELISA Kit
4-SEA797Ra
  • EUR 4677.00
  • EUR 2471.00
  • EUR 627.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Angiotensinogen (AGT) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Angiotensinogen (AGT) Polyclonal Antibody (Rat)
4-PAA797Ra01
  • EUR 243.00
  • EUR 2457.00
  • EUR 613.00
  • EUR 305.00
  • EUR 212.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Angiotensinogen (AGT)

Rat angiotensinogen(aGT)ELISA Kit
QY-E10987 96T
EUR 361

Rat angiotensinogen,aGT ELISA Kit
CN-01790R1 96T
EUR 447

Rat angiotensinogen,aGT ELISA Kit
CN-01790R2 48T
EUR 296

Angiotensinogen (AGT) Antibody
20-abx301357
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Angiotensinogen (AGT) Antibody
20-abx128540
  • EUR 398.00
  • EUR 133.00
  • EUR 1107.00
  • EUR 537.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiotensinogen (AGT) Antibody
20-abx110982
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Angiotensinogen Rabbit mAb
A11689-100ul 100 ul
EUR 410

Angiotensinogen Rabbit mAb
A11689-200ul 200 ul
EUR 571

Angiotensinogen Rabbit mAb
A11689-20ul 20 ul
EUR 221

Angiotensinogen Rabbit mAb
A11689-50ul 50 ul
EUR 287

Angiotensinogen (AGT) Antibody
20-abx101137
  • EUR 398.00
  • EUR 133.00
  • EUR 1107.00
  • EUR 537.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiotensinogen (AGT) Antibody
20-abx101138
  • EUR 411.00
  • EUR 133.00
  • EUR 1135.00
  • EUR 551.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiotensinogen (AGT) Antibody
20-abx101139
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Angiotensinogen (AGT) Antibody
20-abx101140
  • EUR 328.00
  • EUR 815.00
  • EUR 425.00
  • EUR 154.00
  • EUR 258.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Angiotensinogen (AGT) Antibody
abx010360-100ul 100 ul
EUR 411

Angiotensinogen (AGT) Antibody
20-abx004798
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Angiotensinogen (AGT) Antibody
abx033720-400ul 400 ul
EUR 523

Angiotensinogen (AGT) Antibody
abx033720-80l 80 µl
EUR 286

Angiotensinogen (AGT) Antibody
abx033721-400ul 400 ul
EUR 523

Angiotensinogen (AGT) Antibody
abx033721-80l 80 µl
EUR 286

Angiotensinogen (1- 13)
5-00696 4 x 5mg Ask for price

Angiotensinogen (AGT) Antibody
20-abx171240
  • EUR 787.00
  • EUR 411.00
  • 1 mg
  • 200 ug

Angiotensinogen (AGT) Antibody
20-abx320213
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody
20-abx322250
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody
abx230393-100ug 100 ug
EUR 509

Angiotensinogen (AGT) Antibody
abx230394-100ug 100 ug
EUR 481

Angiotensinogen (AGT) Antibody
abx230395-100ug 100 ug
EUR 481

Angiotensinogen (AGT) Antibody
20-abx339646
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody
20-abx241726
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody
20-abx241946
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody
20-abx241947
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody
20-abx242324
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody
20-abx214416
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Angiotensinogen (AGT) Antibody
20-abx175400
  • EUR 1135.00
  • EUR 551.00
  • 1 mg
  • 200 ug

Angiotensinogen (AGT) Antibody
20-abx211344
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Human Angiotensinogen Antibody
11013-05011 150 ug
EUR 217

Angiotensinogen Conjugated Antibody
C49159 100ul
EUR 397

Human Angiotensinogen (AGT)
1-CSB-EP001463HU1
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Angiotensinogen(AGT),partial expressed in E.coli

Eukaryotic Angiotensinogen (AGT)
4-EPA797Mu61
  • EUR 466.46
  • EUR 228.00
  • EUR 1474.24
  • EUR 558.08
  • EUR 1016.16
  • EUR 375.00
  • EUR 3535.60
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Angiotensinogen expressed in: 293F cell

Eukaryotic Angiotensinogen (AGT)
4-EPA797Ra61
  • EUR 471.84
  • EUR 229.00
  • EUR 1494.40
  • EUR 564.80
  • EUR 1029.60
  • EUR 379.00
  • EUR 3586.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Angiotensinogen expressed in: 293F cell

anti- Angiotensinogen antibody
FNab00393 100µg
EUR 548.75
Description: Antibody raised against Angiotensinogen

anti- Angiotensinogen antibody
FNab00394 100µg
EUR 505.25
Description: Antibody raised against Angiotensinogen

anti- Angiotensinogen antibody
FNab00395 100µg
EUR 505.25
Description: Antibody raised against Angiotensinogen

Angiotensinogen, Human Plasma
ATN15-N-100 100 ug
EUR 225

Anti-Angiotensinogen antibody
PAab00393 100 ug
EUR 386

Anti-Angiotensinogen antibody
PAab00394 100 ug
EUR 355

ELISA kit for Rat AGT (Angiotensinogen)
E-EL-R0199 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Rat AGT (Angiotensinogen) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Rat AGT (Angiotensinogen)
E-CL-R0138 1 plate of 96 wells
EUR 584
Description: A sandwich CLIA kit for quantitative measurement of Rat AGT (Angiotensinogen) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Rat AGT (Angiotensinogen)
ELK4678 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Angiotensinogen from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Angiotensinogen (AGT) Monoclonal Antibody (Rat), APC
4-MAA797Ra21-APC
  • EUR 345.00
  • EUR 3275.00
  • EUR 912.00
  • EUR 440.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Rat Angiotensinogen (AGT). This antibody is labeled with APC.

Angiotensinogen (AGT) Monoclonal Antibody (Rat), Biotinylated
4-MAA797Ra21-Biotin
  • EUR 311.00
  • EUR 2460.00
  • EUR 727.00
  • EUR 381.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Rat Angiotensinogen (AGT). This antibody is labeled with Biotin.

An alanine-substitution scanning assay and sequence conservation evaluation elucidated that these porcine mAbs acknowledged two conserved epitopes on VP2: a linear epitope  in the N terminus and a conformational epitope involving residues Ok63, H65, L66, F67, D68 and L81 on two β-sheets (B-sheet and C-sheet) that trusted the integrity of VP2. Random parings of heavy and mild chains of the IgGs confirmed that the heavy chain is predominantly concerned in binding to antigen. The mild chain of porcine IgG contributes to the binding affinity towards an antigen and might operate as a help platform for antibody stability. In abstract, this research is the first to disclose the conserved antigenic profile of FMDV acknowledged by porcine B cells and offers a novel technique for analysing the antibody response in opposition to FMDV in its pure hosts (i.e. pigs) at the clonal stage.

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